We narrowed to 4,591 results for: Ado;
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Plasmid#172596PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.DepositorInsertmyc-PDGFRA-R914W (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-R914WPromoterCMVAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)
Plasmid#156416Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseZebrafish transgenesisTagsmScarlet-IMutationmutant KRAS4A-G12VPromoterkdrlAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 PSMB5 guide 1
Plasmid#117073Purposesingle guide RNA targeting PSMB5; guide 1DepositorAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 C3_20
Plasmid#60303PurposeThis plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.DepositorAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11(R346C)
Plasmid#164206PurposeExpresses CRY2oligo-mcherry tagged ANXA11 R346C mutant under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
TagsCRY2oligo-mcherryExpressionMammalianMutationR346CPromoterCMVAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)
Plasmid#180468Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorAvailable SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 C3_33
Plasmid#60312PurposeThis plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.DepositorAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)
Plasmid#156406PurposeGateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codonDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseGateway cloningTagsmScarlet-IMutationmutant KRAS4A-G12VAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-F-Ova-PuroR [M1G]
Plasmid#171813PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]DepositorInsertmScarlet-F-Ova-T2A-PuroR
UseGene taggingMutationPuroR: Changed Methionin 1 to Glycine.Available SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)
Plasmid#156414Purposekdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseZebrafish transgenesisTagsEGFPMutationmutant KRAS4B-G12VPromoterkdrlAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 PSMB5 guide 2
Plasmid#117074Purposesingle guide RNA targeting PSMB5; guide 2DepositorAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GSDMD_1-270
Plasmid#218229PurposeGasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosisDepositorInsertGSDMD (GSDMD Human)
TagsflagExpressionMammalianMutationconstruct only codes for amino acids 1-270Available SinceJuly 1, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-GSDMD_1-275
Plasmid#218230PurposeGasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosisDepositorInsertGSDMD (GSDMD Human)
TagsflagExpressionMammalianMutationconstruct only codes for amino acids 1-275Available SinceJuly 1, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-GSDMD_29-484
Plasmid#218231PurposeGasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosisDepositorInsertGSDMD (GSDMD Human)
TagsflagExpressionMammalianMutationconstruct only codes for amino acids 29-484Available SinceJuly 1, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-GSDMD_29-270
Plasmid#218232PurposeGasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosisDepositorInsertGSDMD (GSDMD Human)
TagsflagExpressionMammalianMutationconstruct only codes for amino acids 29-270Available SinceJuly 1, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28 MBP-TEV-Rat BCKDK-His
Plasmid#232119PurposeFor bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.DepositorInsertBCKDK (Bckdk Rat)
Tags6 x His, Maltose-Binding Protein (MBP), and Tev S…ExpressionBacterialPromoterT7 PromoterAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-PB2_Lock1-I436C+S993C
Plasmid#182879PurposeLentivirus for expression of Plexin-B2 locked ring mutantDepositorInserthPLXNB2 (PLXNB2 Human)
UseLentiviralMutationchanged Isoleucine 436 to Cysteine and Serine 993…Available SinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-PB2_Lock2-I436C+T1051C
Plasmid#182880PurposeLentivirus for expression of Plexin-B2 locked ring mutantDepositorInserthPLXNB2 (PLXNB2 Human)
UseLentiviralMutationchanged Isoleucine 436 to Cysteine and Threonine …Available SinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter
Plasmid#172598PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.DepositorInsertmyc-PDGFRA-D842V (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842VPromoterCMVAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter
Plasmid#172599PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.DepositorInsertmyc-PDGFRA-D842V+R914W (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842V+R914WPromoterCMVAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only