We narrowed to 2,390 results for: D10

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mTet1 flox donor

Plasmid

#117147

Purpose

Making Tet1 conditional knock out

Depositor

Jacob Hanna

Article

Zviran et al ssrn.3155731

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9-p300 Core

Plasmid

#61357

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Insert

S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)

Use

CRISPR and Synthetic Biology

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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dRED

Plasmid

#139836

Purpose

Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

Depositor

Karim Mekhail

Article

Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

Insert

dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))

Expression

Mammalian

Mutation

RNase H1 protein contains D210N mutation. dCas9 …

Promoter

tet-inducible CMV

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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RED

Plasmid

#139835

Purpose

Expresses RNase H1-EGFP- dCas9 fusion protein in human cells

Depositor

Karim Mekhail

Article

Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

Insert

RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))

Expression

Mammalian

Mutation

dCas9 protein contains two mutations: D10A and H8…

Promoter

tet-inducible CMV

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpG-P2A-EGFP (BKS777/AHK571)

Plasmid

#223124

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd-SpG with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadCBEd-SpG-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpG=D10A/D1135L/S1136W/G1218…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9-p300 Core (D1399Y)

Plasmid

#61358

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Insert

S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)

Use

CRISPR and Synthetic Biology

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pdCas9-DNMT3A-EGFP (ANV)

Plasmid

#71685

Purpose

Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Synthetic, Human)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

CBh

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-DD-KNL1-rvsf_aaaa-dCas9

Plasmid

#248565

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

DD-KNL1-rvsf_aaaa-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 RVSF58-61AAAA; Cas9 D10A, H840A

Promoter

CMV, TetO2

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

Plasmid

#223125

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas9-DNMT3A-PuroR (ANV)

Plasmid

#71684

Purpose

Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Synthetic, Human)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

CBh

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

Plasmid

#223113

Purpose

pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X Y381H

Plasmid

#225723

Purpose

Transfection of USP27X (Y381H) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X Y381H (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

Y381H

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9-p300 Core (Y1467F)

Plasmid

#61362

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Insert

S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)

Use

CRISPR and Synthetic Biology

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

Plasmid

#223114

Purpose

pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas9-DNMT3A-PuroR (ANV)_v2

Plasmid

#74408

Purpose

Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Synthetic, Human)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

CBh

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

Plasmid

#187844

Purpose

Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

IFNAR1 (IFNAR1 Human)

Tags

Ig k-chain leader sequence, SNAPf-tag, and meGFPe…

Expression

Mammalian

Mutation

meGFPe: asparagine 198 to aspartic acid and tyros…

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)

Plasmid

#208293

Purpose

CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA inlaid into nSpRY(D10A/A6…

Promoter

CMV and T7

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

Plasmid

#223115

Purpose

pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

Plasmid

#223116

Purpose

pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

Plasmid

#192785

Purpose

Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

IFNAR1 (IFNAR1 Human)

Tags

Ig k-chain leader sequence, SNAPf-tag, and mXFPe

Expression

Mammalian

Mutation

mXFPe: tyrosine 66 to phenylalanine, asparagine 1…

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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