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pKHH030_sgAMBRA1#2

Plasmid

#174147

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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px458-AMBRA1 (optimized for N-terminal knock-in)

Plasmid

#172608

Purpose

Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

gRNA against AMBRA1 (AMBRA1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2ABFP-W

Plasmid

#163170

Purpose

Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

YAP1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2AmCherry-W

Plasmid

#163172

Purpose

Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

YAP1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Kmt2c-3

Plasmid

#162533

Purpose

sgRNA targeting Kmt2c

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Kmt2c (Kmt2c Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Kmt2b-3

Plasmid

#162537

Purpose

sgRNA targeting Kmt2b

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Kmt2b (Kmt2b Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgCh2-2

Plasmid

#125767

Purpose

constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgCh2-2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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DDR1 gRNA (BRDN0001145659)

Plasmid

#78010

Purpose

3rd generation lentiviral gRNA plasmid targeting human DDR1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DDR1 (DDR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CSNK1E gRNA (BRDN0001147706)

Plasmid

#77978

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1E

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1E,LOC400927-CSNK1E (CSNK1E, TPTEP2-CSNK1E Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GCK gRNA (BRDN0001147104)

Plasmid

#77583

Purpose

3rd generation lentiviral gRNA plasmid targeting human GCK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GCK (GCK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2D gRNA (BRDN0001146190)

Plasmid

#76032

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2D

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2D (GUCY2D Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3b

Plasmid

#180433

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Available since

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3c

Plasmid

#180434

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Available since

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3d

Plasmid

#180435

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Available since

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRNA(CTRL)-CMV-eGFP

Plasmid

#194017

Purpose

Expresses a gRNA that targets the LacZ gene (serves as control) and eGFP

Depositor

Larry Young

Article

Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

Inserts

sgRNA(CTRL)
eGFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A

Plasmid

#52505

Purpose

mammalian expression vector of 53BP1 28A mutant

Depositor

Daniel Durocher

Article

Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

Insert

53BP1 28A (TP53BP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

All 28 SQ/TQ motifs in N-term of 53BP1 have been …

Promoter

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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PGL4.11-COL1A1mTEAD

Plasmid

#230916

Purpose

Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)

Use

Luciferase

Tags

Luciferase- luc2p

Expression

Bacterial and Mammalian

Mutation

mutant potential TEAD bind elements AGGAAT to CTG…

Promoter

COL1A1

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

Plasmid

#202555

Purpose

Synaptotagmin-1 sgRNA2 with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Expression

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

Plasmid

#202556

Purpose

Synaptotagmin-1 sgRNA3 with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Expression

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD 3E@3R

Plasmid

#188149

Purpose

Expresses C-terminal flag-tagged human CAD 3E@3R in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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