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pTRK669

Plasmid

#71313

Purpose

Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

Depositor

Todd Klaenhammer, Rodolphe Barrangou

Article

Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

Type

Empty backbone

Use

E. coli-gram positive bacteria shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pST-KT

Plasmid

#44561

Purpose

for ectopic inducible expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Mutation

Promoter

UV15 promoter

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pYHY3

Plasmid

#216109

Purpose

aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.

Depositor

Shannon Sirk

Article

Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

Insert

NanoLuc

Use

Synthetic Biology; E. coli-bacteroides shuttle ve…

Tags

3xFLAG tag and BT3630 signal peptide

Expression

Bacterial

Mutation

Promoter

P1TDP-A21

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pST-K

Plasmid

#44560

Purpose

for ectopic constitutive expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Mutation

Promoter

UV15 promoter

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pLX-TRV1

Plasmid

#180515

Purpose

Agrobacterium tumefaciens-based expression of TRV1

Depositor

Jose-Antonio Daros

Article

Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

Insert

Tobacco rattle virus RNA1

Use

Tags

Expression

Plant

Mutation

Promoter

35S CaMV

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMGA-ptac-sfGFP

Plasmid

#139934

Purpose

IPTG-inducible sfGFP expression on M. magneticum/E. coli shuttle vector

Depositor

Christopher Voigt

Article

Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

Insert

LacI-Ptac-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac-M2

Plasmid

#135583

Purpose

High eukaryotic protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Article

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Tags

Expression

Insect

Mutation

Promoter

polyhedrin of AcMNPV

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-iRIS

Plasmid

#102584

Purpose

Regulation of protein localization (near-infrared-blue-light-inducible shuttle)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

Insert

BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ265_OoA3GX2

Plasmid

#225147

Purpose

Gateway entry clone (attL1 & attR5) OoA3GX2-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

OoA3GX2-zCas9(D10A)-UGI

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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p201G Cas9

Plasmid

#59178

Purpose

Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
sGFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

2x35S

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pBSV2_PflaB-mCherryBb

Plasmid

#118243

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2_P0826-mCherryBb

Plasmid

#118242

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0826 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0826 promoter driven mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFTK094-LM-AMA002.0

Plasmid

#171366

Purpose

Entry Vector compatible, fungal replicating, shuttle backbone vector from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDD143

Plasmid

#119880

Purpose

Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_dCas9sth1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJYS2cm_crtYf

Plasmid

#85543

Purpose

Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Insert

crRNA of crtYf

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfGFPBb

Plasmid

#118231

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mCherryBb

Plasmid

#118230

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p201B Cas9

Plasmid

#59177

Purpose

Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
BAR

Use

CRISPR

Tags

Expression

Mutation

Promoter

2x35S

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pMGB

Plasmid

#139932

Purpose

M. magneticum/E. coli shuttle vector (partial sequence of pMGT and p15A)

Depositor

Christopher Voigt

Article

Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23101

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pYHY2

Plasmid

#216108

Purpose

Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.

Depositor

Shannon Sirk

Article

Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

Insert

NanoLuc

Use

Synthetic Biology; E. coli-bacteroides shuttle ve…

Tags

3xFLAG tag and BT0294 signal peptide

Expression

Bacterial

Mutation

Promoter

BfP1E6 promoter

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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