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pAG8H-GB1-EWS_171-264

Plasmid

#180466

Purpose

Expresses the construct His8-GB1-EWS_171-264 with a TEV cleavage site between GB1 and EWS_171-264

Depositor

David Libich

Article

Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

Insert

EWSR1 (EWSR1 Human)

Tags

GB1 and His8

Expression

Bacterial

Mutation

deleted 1-170, 265-656

Promoter

T7

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAG8H-GFP-EWS_1-120

Plasmid

#180464

Purpose

Expresses the construct His8-GFP-EWS_1-120 with a TEV cleavage site between GFP and EWS_1-120

Depositor

David Libich

Article

Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

Insert

EWSR1 (EWSR1 Human)

Tags

GFP and His8

Expression

Bacterial

Mutation

deleted 121f-656

Promoter

T7

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-As-NF-kB1

Plasmid

#180209

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB1 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB1

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 2-409

Promoter

AdhI

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-Co-RHD

Plasmid

#180207

Purpose

Expresses GAL4 fusion of Capsaspora NF-kB Rel Homology Domain sequences in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-RHD

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 2-581

Promoter

AdhI

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-As-NF-kB1

Plasmid

#180200

Purpose

Expresses FLAG-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB1

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-409

Promoter

CMV

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-BirA(R118G)-HA-MACROD2

Plasmid

#172582

Purpose

For transient expression of C-terminal BirA-tagged MACROD2

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Tags

BirA(R118G)-HA

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-MYC-As-NF-kB2

Plasmid

#180204

Purpose

Expresses MYC-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Tags

MYC-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-502

Promoter

CMV

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-MYC-As-NF-kB1

Plasmid

#180203

Purpose

Expresses MYC-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB1

Tags

MYC-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-409

Promoter

CMV

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-As-NF-kB2

Plasmid

#180210

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB2 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Tags

GAL4 (aa 1-147) fusion protein

Expression

Yeast

Mutation

aa 2-502

Promoter

AdhI

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1351-1588_AE

Plasmid

#148630

Purpose

Bacterial Expression of HsNot1iso1_1351-1588

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsNot1iso1_1351-1588 (CNOT1 Human)

Expression

Bacterial

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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