We narrowed to 3,616 results for: mak

1. pMRXIP-GFP-CERT(PHD)

   Plasmid

   #221746

   Purpose

   Stable expression of GFP-CERT(PHD)in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   CERT (CERT1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 1–116

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pInt-ERCreER

   Plasmid

   #190040

   Purpose

   This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).

   Depositor

   Kayoko Tanaka

   Article

   Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 27408

   Insert

   ERT2-Cre-ERT2 flanked by AAVS1
   

   Use

   CRISPR and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. WT Smarca4-V5 IRES-puro

   Plasmid

   #107058

   Purpose

   Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

   Depositor

   Jerry Crabtree, Courtney Hodges

   Article

   Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-puroR and V5

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CAG

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGST2-GST-TEV-NDP52

   Plasmid

   #187828

   Purpose

   Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NDP52 (CALCOCO2 Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGenLenti V5-NIX

   Plasmid

   #223731

   Purpose

   Stable expression of protein in mammalian cell culture

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   NIX (BNIP3L) (BNIP3L Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

   Plasmid

   #35503

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   hChR2(C128S/D156A)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C128S and D156A

   Promoter

   Ef1a

   Available since

   April 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. MBP-FUS_FL_6E

   Plasmid

   #98655

   Purpose

   Expresses MBP-tagged full length phosphomimetic FUS

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   6 S/T to E

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-FKBP-mEGFP-WIPI2d (R108E/R125E)

   Plasmid

   #223770

   Purpose

   Stable expression and inducible recruitment of protein in mammalian cell culture

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Lentiviral

   Tags

   FKBP-mEGFP

   Expression

   Mutation

   R108E/R125E

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRXIP-GFP-hATG2A

   Plasmid

   #114462

   Purpose

   Expresses human ATG2A with GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

   Insert

   human ATG2A (ATG2A Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   P656R (please see depositors comment below)

   Promoter

   CMV

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas-Cas12m

    Plasmid

    #192274

    Purpose

    Encodes MmCas12m under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmCas12
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23108

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CD3-2A_pMI-LO

    Plasmid

    #153418

    Purpose

    Retroviral (MSCV) expression of all four WT CD3 subunits, co-expressed with LSSmOrange

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    murine CD3 delta-gamma-epsilon-zeta subunits (Cd3d Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. phROSA26-Tet-HA-JSAP1_WT

    Plasmid

    #208772

    Purpose

    Tetracycline inducible expression vector for HA-JSAP1_WT, used as a donor plasmid for HA-JSAP1_WT knock-in at the human ROSA26 locus

    Depositor

    Katsuji Yoshioka

    Article

    Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

    Insert

    JSAP1 (MAPK8IP3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1A-LivePAR(Y107A)-Hygro

    Plasmid

    #176073

    Purpose

    EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    LivePAR (Y107A)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation to convert Try107 to Ala

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid

    #35498

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    ChR1-VChR1 Chimera
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E122T and E162T

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRXIB-mRuby3-CERT(PHD)

    Plasmid

    #221762

    Purpose

    Stable expression of mRuby3-CERT(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    CERT (CERT1 Human)
    

    Use

    Retroviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    amino acids 1–116

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. K785R Smarca4-sfGFP

    Plasmid

    #107057

    Purpose

    Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

    Depositor

    Jerry Crabtree, Courtney Hodges

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    Super-folder GFP (sfGFP)

    Expression

    Mammalian

    Mutation

    K785R

    Promoter

    CAG

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-TRE-FH-TET3 short

    Plasmid

    #166916

    Purpose

    Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

    Depositor

    Roland Friedel

    Article

    Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

    Insert

    TET3 short isoform
    

    Use

    Lentiviral

    Tags

    HA Tag

    Expression

    Mammalian

    Mutation

    Changed nucleotide 18 (G) to (A) to delete FseI r…

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183

    Plasmid

    #164827

    Purpose

    Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Inserts

    AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    miniCMV and pGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Medium_SynNotch_RHL144

    Plasmid

    #164828

    Purpose

    Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Medium Gal4VP64 Synthetic Notch

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Inserts

    AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    AntiHER2 4D5-Medium Gal4VP64 synthetic Notch receptor
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    miniCMV and pGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSaCas9-1xms2-2x3’UTR

    Plasmid

    #122946

    Purpose

    For expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR

    Depositor

    Baisong Lu

    Article

    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

    Insert

    SaCas9-MS2-HBB 3' UTR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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