3,636 results

1. pAc5.1B-EGFP-Dm4E-T-L36AL39A_T

   Plasmid

   #147604

   Purpose

   Insect Expression of Dm4E-T-L36AL39A

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

   Insert

   Dm4E-T-L36AL39A (4E-T Fly)
   

   Expression

   Insect

   Mutation

   Two non silent mutations (H628R and N659S) compar…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmNot3-dsRNAres_Q

   Plasmid

   #147279

   Purpose

   Insect Expression of DmNot3-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmNot3-dsRNAres (Not3 Fly)
   

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-lambdaN-HA-DmeIF4Ga-Y804A-dsRNAres_Q

   Plasmid

   #147274

   Purpose

   Insect Expression of DmeIF4Ga-Y80A-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmeIF4Ga-Y80A-dsRNAres (eIF4G1 Fly)
   

   Expression

   Insect

   Mutation

   one non silent mutation K340R compared to the seq…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q

   Plasmid

   #147262

   Purpose

   Insect Expression of DmNot3-F818A-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmNot3-F818A-dsRNAres (Not3 Fly)
   

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-HA-DmGW182-10xmut_G

   Plasmid

   #146428

   Purpose

   Insect Expression of DmGW182-10xmut

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

   Insert

   DmGW182-10xmut (gw Fly)
   

   Expression

   Insect

   Mutation

   Multiple mutations compared to NM_166780.1, see a…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P

   Plasmid

   #147209

   Purpose

   Insect Expression of DmXRN1-P1339AP1342A-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   DmXRN1-P1339AP1342A-dsRNAres (pcm Fly)
   

   Expression

   Insect

   Mutation

   one silent mtuation compared to the sequence give…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J

   Plasmid

   #146702

   Purpose

   Insect Expression of DmNot1_1710-2505

   Depositor

   Elisa Izaurralde

   Article

   Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

   Insert

   DmNot1_1710-2505 (Not1 Fly)
   

   Expression

   Insect

   Mutation

   two non silent mutations (A1747T, G1999S) and one…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5.1B-EGFP-DmCup-Y342AL347A_O

   Plasmid

   #147103

   Purpose

   Insect Expression of DmCup-Y342AL347A

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

   Insert

   DmCup-Y342AL347A (cup Fly)
   

   Expression

   Insect

   Mutation

   Y342A, L347A, I350L, P418S, K1045N

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-EGFP-DmHPat-dsRNAres_J

   Plasmid

   #146692

   Purpose

   Insect Expression of DmHPat-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

   Insert

   DmHPat-dsRNAres (Patr-1 Fly)
   

   Expression

   Insect

   Mutation

   two non silent, A479V, G568D and one silent mutat…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1A-DmXRN1-D207A-dsRNAres-V5His6_O

    Plasmid

    #147065

    Purpose

    Insect Expression of DmXRN1-D207A-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmXRN1-D207A-dsRNAres (pcm Fly)
    

    Expression

    Insect

    Mutation

    one silent mtuation compared to the sequence give…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1A-DmXRN1-del1323-1355-dsRNAres-V5His6_N

    Plasmid

    #146969

    Purpose

    Insect Expression of DmXRN1-del1323-1355-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmXRN1-del1323-1355-dsRNAres (pcm Fly)
    

    Expression

    Insect

    Mutation

    one silent mtuation compared to the sequence give…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-lambdaN-HA-DmGW182-delCterm_E

    Plasmid

    #146252

    Purpose

    Insect Expression of DmGW182-delC-term

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

    Insert

    DmGW182-delC-term (gw Fly)
    

    Expression

    Insect

    Mutation

    V758A, Q817R, and N853S

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-lambdaN-HA-DmSMG6-siRNAres-V5His6_E

    Plasmid

    #146241

    Purpose

    Insect Expression of DmSMG6-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

    Insert

    DmSMG6-siRNAres (Smg6 Fly)
    

    Expression

    Insect

    Mutation

    T151A, deleted A947

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM-60-NusA-DmGe1_1220-1354_G

    Plasmid

    #146420

    Purpose

    Bacterial Expression of DmGe1_1220-1354

    Depositor

    Elisa Izaurralde

    Article

    Jinek et al RNA. 2008 Oct . 14(10):1991-8.

    Insert

    DmGe1_1220-1354 (Ge-1 Fly)
    

    Expression

    Bacterial

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM-60-NusA-DmEDC3_331-440_H

    Plasmid

    #146448

    Purpose

    Bacterial Expression of DmEDC3_331-440

    Depositor

    Elisa Izaurralde

    Insert

    DmEDC3_331-440 (Edc3 Fly)
    

    Expression

    Bacterial

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-2T-TRR-C421-G2304D

    Plasmid

    #182845

    Purpose

    mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRR 2011-2431 (trr Fly)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    mutation G2304D (GGC/GAC)

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-2T-CBP(1-287)

    Plasmid

    #182839

    Purpose

    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    CBP1-287
    

    Expression

    Bacterial

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-UTX(420-633)-H6

    Plasmid

    #183773

    Purpose

    Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

    Insert

    UTX
    

    Tags

    6 His tag at the C-terminus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET11-PC(75-228)-H6

    Plasmid

    #183779

    Purpose

    Express PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.

    Depositor

    Peter Harte

    Article

    Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

    Insert

    Polycomb (Pc Fly)
    

    Tags

    6 His Tag at the C-terminus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET11-PC(75-390)-H6

    Plasmid

    #183781

    Purpose

    Express PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assays

    Depositor

    Peter Harte

    Article

    Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

    Insert

    Polycomb (w Fly)
    

    Tags

    6 His tag at the C-terminus

    Expression

    Bacterial

    Mutation

    6 His tag at the C-terminus

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge