3,636 results

1. pAc5.1B-lambdaN-HA-DmeIF4Ga-Y804A-dsRNAres_Q

   Plasmid

   #147274

   Purpose

   Insect Expression of DmeIF4Ga-Y80A-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmeIF4Ga-Y80A-dsRNAres (eIF4G1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one non silent mutation K340R compared to the seq…

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q

   Plasmid

   #147262

   Purpose

   Insect Expression of DmNot3-F818A-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmNot3-F818A-dsRNAres (Not3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-lambdaN-HA-DmGW182-10xmut_G

   Plasmid

   #146428

   Purpose

   Insect Expression of DmGW182-10xmut

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

   Insert

   DmGW182-10xmut (gw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Multiple mutations compared to NM_166780.1, see a…

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P

   Plasmid

   #147209

   Purpose

   Insect Expression of DmXRN1-P1339AP1342A-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   DmXRN1-P1339AP1342A-dsRNAres (pcm Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mtuation compared to the sequence give…

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J

   Plasmid

   #146702

   Purpose

   Insect Expression of DmNot1_1710-2505

   Depositor

   Elisa Izaurralde

   Article

   Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

   Insert

   DmNot1_1710-2505 (Not1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two non silent mutations (A1747T, G1999S) and one…

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1B-EGFP-DmCup-Y342AL347A_O

   Plasmid

   #147103

   Purpose

   Insect Expression of DmCup-Y342AL347A

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

   Insert

   DmCup-Y342AL347A (cup Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Y342A, L347A, I350L, P418S, K1045N

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-EGFP-DmHPat-dsRNAres_J

   Plasmid

   #146692

   Purpose

   Insect Expression of DmHPat-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

   Insert

   DmHPat-dsRNAres (Patr-1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two non silent, A479V, G568D and one silent mutat…

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1A-DmXRN1-D207A-dsRNAres-V5His6_O

   Plasmid

   #147065

   Purpose

   Insect Expression of DmXRN1-D207A-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmXRN1-D207A-dsRNAres (pcm Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mtuation compared to the sequence give…

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1A-DmXRN1-del1323-1355-dsRNAres-V5His6_N

   Plasmid

   #146969

   Purpose

   Insect Expression of DmXRN1-del1323-1355-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmXRN1-del1323-1355-dsRNAres (pcm Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mtuation compared to the sequence give…

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-DmGW182-delCterm_E

    Plasmid

    #146252

    Purpose

    Insect Expression of DmGW182-delC-term

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

    Insert

    DmGW182-delC-term (gw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    V758A, Q817R, and N853S

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1B-lambdaN-HA-DmSMG6-siRNAres-V5His6_E

    Plasmid

    #146241

    Purpose

    Insect Expression of DmSMG6-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

    Insert

    DmSMG6-siRNAres (Smg6 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    T151A, deleted A947

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM-60-NusA-DmGe1_1220-1354_G

    Plasmid

    #146420

    Purpose

    Bacterial Expression of DmGe1_1220-1354

    Depositor

    Elisa Izaurralde

    Article

    Jinek et al RNA. 2008 Oct . 14(10):1991-8.

    Insert

    DmGe1_1220-1354 (Ge-1 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM-60-NusA-DmEDC3_331-440_H

    Plasmid

    #146448

    Purpose

    Bacterial Expression of DmEDC3_331-440

    Depositor

    Elisa Izaurralde

    Insert

    DmEDC3_331-440 (Edc3 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-2T-TRR-C421-G2304D

    Plasmid

    #182845

    Purpose

    mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRR 2011-2431 (trr Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    mutation G2304D (GGC/GAC)

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-2T-CBP(1-287)

    Plasmid

    #182839

    Purpose

    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    CBP1-287
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-UTX(420-633)-H6

    Plasmid

    #183773

    Purpose

    Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

    Insert

    UTX
    

    Use

    Tags

    6 His tag at the C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET11-PC(75-228)-H6

    Plasmid

    #183779

    Purpose

    Express PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.

    Depositor

    Peter Harte

    Article

    Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

    Insert

    Polycomb (Pc Fly)
    

    Use

    Tags

    6 His Tag at the C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET11-PC(75-390)-H6

    Plasmid

    #183781

    Purpose

    Express PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assays

    Depositor

    Peter Harte

    Article

    Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

    Insert

    Polycomb (w Fly)
    

    Use

    Tags

    6 His tag at the C-terminus

    Expression

    Bacterial

    Mutation

    6 His tag at the C-terminus

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-PC(1-86)-H6

    Plasmid

    #183780

    Purpose

    Express PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assays

    Depositor

    Peter Harte

    Article

    Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

    Insert

    Polycomb (Pc Fly)
    

    Use

    Tags

    6 His Tag at the C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-SU(Z)12(624-900)-H6

    Plasmid

    #183772

    Purpose

    Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

    Insert

    SU(Z)12 residues 624-900 (Su(z)12 Fly)
    

    Use

    Tags

    His tag at the C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge