Addgene

Showing: 2321 - 2340 of 7300 results

Results per page:

Sort results by:

pPD033 Reporter Template v1 (Far Spaced)

Plasmid

#138754

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcGP67B human Fzd4 CRD (38–160)

Plasmid

#132621

Purpose

expresses human Fzd4 CRD (38-160) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Shen et al Cell Res. 2015 Sep;25(9):1078-81. doi: 1

Insert

Fzd4 CRD (38–160) (FZD4 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM170/171

Plasmid

#84097

Purpose

target miRNA170/171 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM170/171

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-EsaR-I70V/D91G/V220A

Plasmid

#47647

Purpose

pAC-EsaR with a208g, a272g, c659t mutations in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-I70V/D91G/V220A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Changed Isoleucine 70 to Valine, Aspartic acid 91…

Promoter

Plac

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Cerulean-Epac2(R53A)-Venus

Plasmid

#138197

Purpose

FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R53A)-Venus (Rapgef4 Mouse, Synthetic)

Use

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Cerulean-Epac2(R466A)-Venus

Plasmid

#138199

Purpose

FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R466A)-Venus (Rapgef4 Mouse, Synthetic)

Use

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Cerulean-Epac2(L426W)-Venus

Plasmid

#138200

Purpose

FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)

Use

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains leucine-to-tryptophan substitution at re…

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

Plasmid

#126705

Purpose

donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

GFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 LRP6-APEX2-Ires-eGFP-PAC

Plasmid

#180142

Purpose

used to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-Pink Flamindo-p2a-bPAC

Plasmid

#202716

Purpose

Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Inserts

Pink Flamindo
bPAC

Use

Lentiviral

Tags

10xHis and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4

Plasmid

#214137

Purpose

Insect cell expression of the human 20SCP proteasome assembly chaperones

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

POMP (POMP Human)
PSMG1 (PSMG1 Human)
PSMG2 (PSMG2 Human)
PSMG3 (PSMG3 Human)
PSMG4 (PSMG4 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
attB53-Pac-TRE-3xFlag-Neurog1-attB53

Plasmid

#183613

Purpose

RMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

Neurog1 (Neurog1 Mouse)

Use

Rmce

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SPACA6-COMP5AP-AviTag-9xHis

Plasmid

#157274

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SPACA6 (SPACA6 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

Plasmid

#62044

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

LAP2beta (Tmpo Rat)

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

Plasmid

#118274

Purpose

Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Inserts

bPAC(WT)
SthK

Use

AAV

Tags

His tag, P2A, mCherry, and myc tag

Expression

Mammalian

Mutation

Promoter

CamK II

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII

Plasmid

#217896

Purpose

Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tag

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMB5 (PSMB5 Human)
PSMB6 (PSMB6 Human)
PSMB7 (PSMB7 Human)

Use

Tags

2xSTII

Expression

Insect

Mutation

Promoter

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM165/166-48nt

Plasmid

#84091

Purpose

target miRNA165/166 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM165/166

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)

Plasmid

#165489

Purpose

Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus-bPAC(F198Y)

Use

Cdna expression, xenopus oocyte

Tags

Venus

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG

Plasmid

#167477

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(K42M)-ShadowG

Use

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG

Plasmid

#167476

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(T286A)-ShadowG

Use

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge