163,682 results

1. EGFP-p300

   Plasmid

   #191764

   Purpose

   Expression of EGFP fused to core p300 histone acetyltransferase

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   FRB-EGFP-p300 core (EP300 Human)
   

   Use

   Tags

   EGFP (N-terminal of p300 core) and FRB (N-termina…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROP_C9_Puro_EPHA2

   Plasmid

   #183286

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets EPHA2 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   EPHA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-syn-jGCaMP7f-WPRE

   Plasmid

   #104488

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9

   Insert

   jGCaMP7f
   

   Use

   AAV

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pM-ABE

   Plasmid

   #196292

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding ABE in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRPF215

   Plasmid

   #106377

   Purpose

   C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

   Depositor

   Robert Fagan, Neil Fairweather

   Article

   Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

   Insert

   Ptet-himar1-Ter(slpA)-transposon
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Codon optimised for C. difficile

   Promoter

   Ptet

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. His 3C MKK6 1-334

   Plasmid

   #87881

   Purpose

   E. coli expression of MKK6 aa 1-334

   Depositor

   Peter Mace

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Tags

   3C protease site and 6xHis

   Expression

   Bacterial

   Mutation

   aa 1-334

   Promoter

   T7

   Available Since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-nanoHER2-SpyTag003

   Plasmid

   #216312

   Purpose

   Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   nanoHER2-SpyTag003
   

   Use

   Tags

   His6 tag and SpyTag003

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1a-eFFly-eGFP

   Plasmid

   #104834

   Purpose

   eFFly luciferase for invivo Imaging, eGFP for flourescent detection

   Depositor

   Irmela Jeremias

   Article

   Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

   Inserts

   eFFly
   eGFP
   

   Use

   Lentiviral and Luciferase; Egfp

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOpen-lambda red operon

   Plasmid

   #165521

   Purpose

   Operon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   lambda red operon
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 3, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Lenti-EF1alpha-scFv-p65-HSF1-Blast

    Plasmid

    #192652

    Purpose

    3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    scFv-p65-HSF1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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