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pNK189

Plasmid

#219743

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiens

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal hispidin-3-hydroxylase

Use

Synthetic Biology

Tags

Expression

Mutation

D37E, V181I, S323M, M385K

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK4169

Plasmid

#219744

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal hispidin-3-hydroxylase

Use

Synthetic Biology

Tags

Expression

Mutation

D37E, V181I, S323M, M385K

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7183

Plasmid

#219760

Purpose

MoClo-compatible Level 1 for expression PzPKS2 in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PzPKS2

Use

Tags

Expression

Plant

Mutation

Promoter

p35s_0.4kb - 5'UTR TMV omega

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7187

Plasmid

#219761

Purpose

MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Use

Tags

Expression

Plant

Mutation

Promoter

p35s_0.4kb - 5'UTR TMV omega

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7180

Plasmid

#219762

Purpose

MoClo-compatible Level 1 for expression HmS in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

HmS

Use

Tags

Expression

Plant

Mutation

Promoter

p35s_0.4kb - 5'UTR TMV omega

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAVS(GMIP pLxIS)

Plasmid

#221316

Purpose

Expression of MAVS chimera with GMIP pLxIS domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS chimera with GMIP pLxIS domain (Mavs Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAVS(pLxIS trunc)

Plasmid

#221314

Purpose

Expression of MAVS truncated pLxIS domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS truncated pLxIS domain (Mavs Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-MAVS(trunc Cterm)

Plasmid

#221259

Purpose

Expression of MAVS truncation (393-431) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS truncation (393-431) (Mavs Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(pLxIS) S365A

Plasmid

#221241

Purpose

Expression of mutant STING CTT in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING CTT (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

S356A

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(pLxIS) L373A

Plasmid

#221242

Purpose

Expression of mutant STING CTT in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING CTT (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

L373A

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83-GB1_AD

Plasmid

#148514

Purpose

Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD

Plasmid

#148515

Purpose

Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-RBMS3

Plasmid

#185557

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting RBMS3

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

RBMS3 gRNAs

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-HHIP

Plasmid

#185551

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting HHIP

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

HHIP gRNAs

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-ADGRG6

Plasmid

#185554

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting ADGRG6

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

ADGRG6 gRNAs

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph18_eGFP_CCR5TC

Plasmid

#187443

Purpose

Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph18

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph15_eGFP_CCR5TC

Plasmid

#187442

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Pet22b-YAPL318E

Plasmid

#166444

Purpose

Bacterial expression of 6x His tagged YAPL318E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAPL318E (YAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-YAP4LE

Plasmid

#166445

Purpose

Bacterial expression of 6x His tagged YAP4LE

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP4LE (YAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Donor-CD55-NeoR

Plasmid

#153551

Purpose

Used as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassette

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

mutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms

Use

AAV and Cre/Lox; Donor plasmid/targeting vector

Tags

N/A

Expression

Mutation

a 1-bp mutation in exon 4

Promoter

No promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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