We narrowed to 10,315 results for: plasmids 101

1. pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

   Plasmid

   #186993

   Purpose

   AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-s1006-gggs-d122, non-targeting crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   s1006-gggs-d1221 deletion

   Promoter

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

   Plasmid

   #102797

   Purpose

   Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Inserts

   sgRNA
   EGFP
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   H231L

   Promoter

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc

   Plasmid

   #186987

   Purpose

   AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guide

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-s1006-gggs-d122, Gluc crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   s1006-gggs-d1221 deletion

   Promoter

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA4TO-3XFLAG Halo WT nsp1

   Plasmid

   #175412

   Purpose

   Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 nsp1 (ORF1ab CoV2)
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-SARS-CoV-2 Nsp3TMC111A

   Plasmid

   #204207

   Purpose

   Mammalian expression of SARS-CoV-2 Nsp3

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
   

   Use

   Tags

   FLAGx1

   Expression

   Mammalian

   Mutation

   Cys to Ala in active site of PLpro

   Promoter

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. kiCAP-AAV-M.Mus.2

   Plasmid

   #196682

   Purpose

   Rep/Cap plasmid for the production of M.Mus.2, an AAV capsid with CNS tropism in mice.

   Depositor

   Pardis Sabeti

   Article

   Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   REQQKLW insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

   Plasmid

   #186991

   Purpose

   AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-r1045-gggs-r1122, non-targeting crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   r1045-gggs-r1122 deletion

   Promoter

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET30a-SARS-CoV2-NP-Nt(47-173)

   Plasmid

   #165091

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 22, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

   Plasmid

   #205098

   Purpose

   Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. d

   Insert

   mNeonGreen, eKL9h, and Volvox channelrhodopsin-1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-TRS-Leader-SARS-CoV-2-EGFP

    Plasmid

    #171585

    Purpose

    Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporter

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    TRS-Leader-SARS-CoV-2-d2eGFP
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET32a-Spike-S-4P

    Plasmid

    #165094

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

    Plasmid

    #175421

    Purpose

    Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET30a-SARS-CoV2-ORF3b (S23Q)

    Plasmid

    #165088

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Stop codon to glutamine at 23rd amino acid

    Promoter

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pJS699

    Plasmid

    #168779

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD (S betacoronavirus)
    

    Use

    Tags

    Cmyc

    Expression

    Mutation

    N343Q

    Promoter

    GAL1

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET32a-Spike-403-505

    Plasmid

    #165095

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pETDuet-1-6xHis-TEV-Nsp8

    Plasmid

    #201022

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PAK4 gRNA (BRDN0001147629)

    Plasmid

    #77573

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PAK4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PAK4 (PAK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PAK4 gRNA (BRDN0001148325)

    Plasmid

    #77576

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PAK4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PAK4 (PAK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PAK4 gRNA (BRDN0001146148)

    Plasmid

    #77574

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PAK4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PAK4 (PAK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PAK4 gRNA (BRDN0001145608)

    Plasmid

    #77575

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PAK4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PAK4 (PAK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge