We narrowed to 8,515 results for: SET

1. pOVC1

   Plasmid

   #131171

   Purpose

   Cloning vector containing A-B-C casette

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHOXC13-donor

   Plasmid

   #113827

   Purpose

   CRISPR donor plasmid to tag human transcription factor HOXC13 with GFP

   Depositor

   Kevin White

   Insert

   HOXC13 homology arms flanking EGFP-IRES-Neo cassette (HOXC13 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGLIS3-donor

   Plasmid

   #113812

   Purpose

   CRISPR donor plasmid to tag human transcription factor GLIS3 with GFP

   Depositor

   Kevin White

   Insert

   GLIS3 homology arms flanking EGFP-IRES-Neo cassette (GLIS3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   rs10118031, rs10973702, rs912257

   Promoter

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSRF-donor

   Plasmid

   #112362

   Purpose

   CRISPR donor plasmid to tag human transcription factor SRF with GFP

   Depositor

   Kevin White

   Insert

   SRF homology arms flanking EGFP-IRES-Neo cassette (SRF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:6:43178470:C>A, hg38:6:43178514:G>A

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHBP1-donor

   Plasmid

   #112336

   Purpose

   CRISPR donor plasmid to tag human transcription factor HBP1 with GFP

   Depositor

   Kevin White

   Insert

   HBP1 homology arms flanking EGFP-IRES-Neo cassette (HBP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZNF785-donor

   Plasmid

   #112317

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF785 with GFP

   Depositor

   Kevin White

   Insert

   ZNF785 homology arms flanking EGFP-IRES-Neo cassette (ZNF785 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:16:30582292:A>T, hg38:16:30582302:A>T,…

   Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZNF354B-donor

   Plasmid

   #112309

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF354B with GFP

   Depositor

   Kevin White

   Insert

   ZNF354B homology arms flanking EGFP-IRES-Neo cassette (ZNF354B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:5:178884427:G>A, hg38:5:178883445:C>T,…

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJet1.2 ftsY

   Plasmid

   #117130

   Purpose

   FtsY gene cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   ftsY
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVKG1-BSD

   Plasmid

   #108680

   Purpose

   Blasticidin resistant cassette for knock-in by VIKING method

   Depositor

   Shigeo Sugano

   Article

   Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

   Insert

   SV40pro-BSD-polyA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. P0050::rpaA

    Plasmid

    #101830

    Purpose

    drives expression of rpaA from an rpaA-independent promoter

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    RpaA with p0050 promoter (+ gentamicin resistance casette)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CpcB-Pinus-banksianaPHLS_CpcA

    Plasmid

    #74002

    Purpose

    Expresses the Pinus banksiana PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

    Depositor

    Anastasios Melis

    Article

    Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

    Insert

    PHLS and CmR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cpc

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CpcB-Picea-sitchensisPHLS_CpcA

    Plasmid

    #74001

    Purpose

    Expresses the Picea sitchensis PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

    Depositor

    Anastasios Melis

    Article

    Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

    Insert

    PHLS and CmR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cpc

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CpcB-Abies-grandisPHLS_CpcA

    Plasmid

    #74000

    Purpose

    Expresses the Abies grandis PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

    Depositor

    Anastasios Melis

    Article

    Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

    Insert

    PHLS and CmR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cpc

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pST50Trc4-CBPNDHFR

    Plasmid

    #63995

    Purpose

    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Calmodulin Binding Peptide (CBP); TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pST50Trc3-HPCHISNDHFR

    Plasmid

    #63984

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis & Protein C (HPC); TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pST50Trc1-HPCHISNDHFR

    Plasmid

    #63948

    Purpose

    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis & Protein C (HPC); TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pST50Trc1-CBPHISNDHFR

    Plasmid

    #63945

    Purpose

    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis & Calmodulin Binding Peptide (CBP); TEV…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pST50Trc3-STRHISNDHFR

    Plasmid

    #63982

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis & Strep II (STR); TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST50Trc3-CBPNDHFR

    Plasmid

    #63977

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Calmodulin Binding Peptide (CBP); TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pST50Trc2-STRHISNDHFR

    Plasmid

    #63964

    Purpose

    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis & Strep II (STR); TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pST50Trc2-CBPHISNDHFR

    Plasmid

    #63963

    Purpose

    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis & Calmodulin Binding Peptide (CBP); TEV…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LMB

    Plasmid

    #12502

    Depositor

    David Baker

    Article

    Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

    Insert

    LMB
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    RosettaDesign was used to design sequences. Align…

    Promoter

    Available since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

23. ACY

    Plasmid

    #12396

    Depositor

    David Baker

    Article

    Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

    Insert

    ACY
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    RosettaDesign was used to design sequences. Align…

    Promoter

    Available since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

24. TEN

    Plasmid

    #12498

    Depositor

    David Baker

    Article

    Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

    Insert

    TEN
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    RosettaDesign was used to design sequences. Align…

    Promoter

    Available since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pCAG-loxPSTOPloxP-ZsGreen

    Plasmid

    #51269

    Purpose

    Cre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by loxP sites followed by ZsGreen

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    loxP-STOP-loxP-ZsGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLVTHM

    Plasmid

    #12247

    Purpose

    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX330-BbsI-PITCh

    Plasmid

    #127875

    Purpose

    For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pACYCDuet-1_Ec_rpoZ

    Plasmid

    #128837

    Purpose

    E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

    Depositor

    Seth Darst

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Insert

    rpoZ (rpoZ E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. px330-mcherry

    Plasmid

    #98750

    Purpose

    Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

    Depositor

    Jinsong Li

    Article

    Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB-CA

    Plasmid

    #20960

    Purpose

    piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSEED-sfGFP

    Plasmid

    #219639

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBTK622

    Plasmid

    #180592

    Purpose

    tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

    Inserts

    tdk
    kanR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFA6a-kanMX6

    Plasmid

    #39296

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAG-roxSTOProx-ZsGreen

    Plasmid

    #51274

    Purpose

    Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    roxSTOProx-ZsGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-U6-gRNA-CBh-mCherry

    Plasmid

    #91947

    Purpose

    Expression of empty gRNA cassette and mCherry

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pARS3_MUbCAS9_GW

    Plasmid

    #128646

    Purpose

    T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

    Depositor

    G. Eric Schaller

    Article

    Worthen et al Development. 2019 Jun 3. pii: dev.174870

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AAVS1-DRGFP

    Plasmid

    #113193

    Purpose

    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    DR-GFP
    

    Use

    Donor construct with t2a-hygromycin for gene trap…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pNBU2_erm-TetR-P1T_DP-GH023

    Plasmid

    #90324

    Purpose

    Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSEED-mScarlet3

    Plasmid

    #219640

    Purpose

    Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mScarlet SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pT2ST

    Plasmid

    #59384

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-dfrA
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBT378_pattB-pCA-GFP-pA-attB

    Plasmid

    #52554

    Purpose

    for PhiC31 integrase-mediated cassette exchange

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLKO-MCS

    Plasmid

    #185594

    Purpose

    Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pDCC6

    Plasmid

    #59985

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Inserts

    Cas9
    U6-2>sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    U6-96Ab and hsp70Bb

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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46. pSEED-ALFA:HA

    Plasmid

    #219646

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    ALFA:HA SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pSEED-sfGFP:ALFA

    Plasmid

    #219642

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP:ALFA SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCXLE-gw

    Plasmid

    #37626

    Purpose

    Non-integrating (episomal) expression vector with Gateway cassette

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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49. pSEED-mGreenLantern

    Plasmid

    #219643

    Purpose

    Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mGreenLantern SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pWH1266-Apra-sgRNA

    Plasmid

    #218372

    Purpose

    sgRNA expression plasmid for CRISPR-intereference

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Insert

    sgRNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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