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Plasmid#187442PurposeEncodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph15
UseAAVTagseGFPExpressionMammalianMutationPromoterSynapsinAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-YAPL318E
Plasmid#166444PurposeBacterial expression of 6x His tagged YAPL318EDepositorInsertYAPL318E (YAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-YAP4LE
Plasmid#166445PurposeBacterial expression of 6x His tagged YAP4LEDepositorInsertYAP4LE (YAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-CD55-NeoR
Plasmid#153551PurposeUsed as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassetteDepositorInsertmutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
UseAAV and Cre/Lox; Donor plasmid/targeting vectorTagsN/AExpressionMutationa 1-bp mutation in exon 4PromoterNo promoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v2-10 (-cyt)
Plasmid#137825Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v2-10 (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic region - N213S on the CD44 re…PromoterPGKAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGA-NeoR
Plasmid#153549PurposeUsed as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassetteDepositorInsertmutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
UseAAV and Cre/Lox; Donor plasmid/targeting vectorTagsN/AExpressionMutationa 3-bp mutation in exon 6PromoterNo promoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-NCOA7g3 (BB36)
Plasmid#139454PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-NCOA7g2 (BB35)
Plasmid#139453PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
p21-P369S-MEFV
Plasmid#134712PurposeLentiviral expression of human MEFV P369S after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.P369SPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p21-M680I-MEFV
Plasmid#134711PurposeLentiviral expression of human MEFV M680I after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.M680IPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p21-M694I-MEFV
Plasmid#134710PurposeLentiviral expression of human MEFV M694I after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.M694IPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p21-S208C/M694V-MEFV
Plasmid#134709PurposeLentiviral expression of human MEFV S208C/M694V after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.S208C/M694VPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p21-M694V-MEFV
Plasmid#134703PurposeLentiviral expression of human MEFV M694V after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.M694VPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-H3
Plasmid#124435PurposeChimeric ETS domain: H3 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis TagExpressionBacterialMutationResidues 225 to 239 replaced with corresponding s…PromoterAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Loop
Plasmid#124551PurposeChimeric ETS domain: Loop of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 216 to 223 replaced with corresponding s…PromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-H3/S3
Plasmid#124627PurposeChimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 237 to 241 replaced with corresponding s…PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-S3
Plasmid#124550PurposeChimeric ETS domain: S3 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 242 to 246 replaced with corresponding s…PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PU.1-ETS-H2
Plasmid#123358PurposeChimeric ETS domain: H2 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 207 to 215 replaced with corresponding s…PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
tdEos-EB3-7
Plasmid#57610PurposeLocalization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertEB3 (MAPRE3 Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS-CD4-YEML-myc
Plasmid#58538PurposeExpresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.DepositorInsertCD4 (CD4 Human)
UseTagsIFITM3 endocytosis Tyrosine-Glutamate-Methionine-…ExpressionMammalianMutationAdded C-terminal Tyrosine-Glutamate-Methionine-Le…PromoterAvailable sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only