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pDONR223-PIM1

Plasmid

#23692

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIM1 (PIM1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PFKL

Plasmid

#23729

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKL (PFKL Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-CRTC1-MAML2

Plasmid

#237638

Purpose

For overexpression of mEGFP-CRTC1-MAML2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CRTC1-MAML2 (CRTC1, MAML2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-CRTC1-MAML2-KS

Plasmid

#237672

Purpose

For overexpression of mEGFP-CRTC1-MAML2-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CRTC1-MAML2-KS (CRTC1, MAML2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Plasmid

#179105

Purpose

pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

Plasmid

#179115

Purpose

Cargo EGFP with AttP Bxb1 site (Parent minicircle)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

Cargo EGFP with AttP Bxb1 site

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-PFKFB3

Plasmid

#23668

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKFB3 (PFKFB3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Plasmid

#179106

Purpose

pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

Plasmid

#179116

Purpose

Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

Cargo EGFP with AttP Bxb1 site for in-frame fusion

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NUP98-HOXA9

Plasmid

#237639

Purpose

For overexpression of mEGFP-NUP98-HOXA9

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NUP98-HOXA9 (NUP98, HOXA9 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-PDK1

Plasmid

#23804

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDK1 (PDK1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDY0663 ACTB atgRNA with v2 scaffold

Plasmid

#179108

Purpose

ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-PDK3

Plasmid

#23556

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDK3 (PDK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-HK2

Plasmid

#23854

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HK2 (HK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PFKP

Plasmid

#23869

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKP (PFKP Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PDPK1

Plasmid

#23801

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDPK1 (PDPK1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PKM2

Plasmid

#23757

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PKM2 (PKM Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK38

Plasmid

#23811

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK38 (STK38 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PCK2

Plasmid

#23715

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PCK2 (PCK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLV-UbC-Tet1v4-dCas9

Plasmid

#232180

Purpose

Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Insert

Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, H840A

Promoter

hUbC

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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