Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,673 results for: pcr

Clear keywords
Showing: 2361 - 2380 of 4673 results

  1. GGE083

    Plasmid
    #120732
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Auxotrophy marker for selection after transformation in Y. lipolytica (Marker_URA3)
    Depositor
    Tristan Rossignol
    Publication
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    Marker_URA3
    Use
    Cloning/assembly plasmid
    Available since
    Oct. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    120732-plasmid-map-sequence-id-259137

    Enlarge

  3. L0I74-_tUBQ10_GG4-GG6

    Plasmid
    #219713
    Purpose
    Level 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-tUBQ10-GG6
    Use
    Synthetic Biology
    Mutation
    WT terminator with added golden gate linkers
    Available since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219713-plasmid-map-sequence-id-435864

    Enlarge

  4. L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4

    Plasmid
    #219709
    Purpose
    Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH:PhiC31:tUBQ1-GG4
    Use
    Synthetic Biology
    Available since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219709-plasmid-map-sequence-id-435862

    Enlarge

  8. pDONR221-kcnj4

    Plasmid
    #173131
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Publication
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj4 (kcnj4 Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173131-plasmid-map-sequence-id-343128

    Enlarge

  11. L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6

    Plasmid
    #219712
    Purpose
    Level 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-pGATA23-Bxb1-tUBQ10-GG6
    Use
    Synthetic Biology
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219712-plasmid-map-sequence-id-435863

    Enlarge

  16. pCCL-c-MNDU3-CD3D-274+5G>A

    Plasmid
    #198023
    Purpose
    LV Expression of CD3D cDNA with mutation at c.274+5G>A used as a disease editing target for CD3d SCID mutation identified in Ecuador
    Depositor
    Donald Kohn
    Insert
    CD3D c.274+5G>A
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    198023-plasmid-map-sequence-id-390930

    Enlarge

  17. pCCL-c-MNDU3-CD3D-275-2A>G

    Plasmid
    #198024
    Purpose
    LV Expression of CD3D cDNA with mutation at c.275-2A>G used as a disease editing target for CD3d SCID mutation identified in Japan
    Depositor
    Donald Kohn
    Insert
    CD3D c.275-2A>G
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198024-plasmid-map-sequence-id-390335

    Enlarge

  19. pENTR-D-kcnj13

    Plasmid
    #173143
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Publication
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj13 (kcnj13 Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173143-plasmid-map-sequence-id-343318

    Enlarge

Showing: 2361 - 2380 of 4673 results