We narrowed to 10,315 results for: plasmids 101

1. MAP3K19 gRNA (BRDN0001146020)

   Plasmid

   #76693

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP3K19

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP3K19 (YSK4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCHC036 (∆pHG1)

   Plasmid

   #226206

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for the pHG1 megaplasmid replication region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   ∆helD ∆repA ∆repB ∆parAB

   Promoter

   Available Since

   Nov. 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pVPSBA01-SARS-CoV2-nsp1

   Plasmid

   #192171

   Purpose

   express SARS2-NSP1

   Depositor

   Sidi Chen

   Article

   Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

   Insert

   express SARS2-NSP1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDF0428 huDisCas7-11-R1045-GGGS-R1122-U6-pro-Gluc

   Plasmid

   #186985

   Purpose

   AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of Gluc crRNA guide

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-r1045-gggs-r1122, Gluc crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   r1045-gggs-r1122 deletion

   Promoter

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)

   Plasmid

   #133796

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized AcrIIA1 (truncated C-terminal domain only)
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   C-terminal domain only

   Promoter

   CMV and T7

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)

   Plasmid

   #133798

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized AcrIIA1(T114A/F115A)
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   T114A/F115A

   Promoter

   CMV and T7

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PFKFB2 gRNA (BRDN0001145863)

   Plasmid

   #77685

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PFKFB2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PFKFB2 (PFKFB2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PFKFB2 gRNA (BRDN0001146926)

   Plasmid

   #77686

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PFKFB2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PFKFB2 (PFKFB2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PFKFB2 gRNA (BRDN0001147260)

   Plasmid

   #77687

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PFKFB2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PFKFB2 (PFKFB2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PFKFB2 gRNA (BRDN0001147256)

    Plasmid

    #77688

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PFKFB2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PFKFB2 (PFKFB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MAP3K19 gRNA (BRDN0001148579)

    Plasmid

    #76694

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP3K19

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP3K19 (YSK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MAP3K19 gRNA (BRDN0001148706)

    Plasmid

    #76695

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP3K19

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP3K19 (YSK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SBK2 gRNA (BRDN0001146871)

    Plasmid

    #75881

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SBK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SBK2 (SBK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SBK2 gRNA (BRDN0001147500)

    Plasmid

    #75882

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SBK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SBK2 (SBK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SBK2 gRNA (BRDN0001162195)

    Plasmid

    #75883

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SBK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SBK2 (SBK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SBK2 gRNA (BRDN0001162192)

    Plasmid

    #75884

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SBK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SBK2 (SBK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. phDLX2-N174

    Plasmid

    #60860

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX2 (DLX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. prhaBAD-anti-mouse-Nb-Hia5

    Plasmid

    #239626

    Purpose

    Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

    Depositor

    Aaron Straight

    Article

    Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

    Insert

    Hia5
    

    Use

    Tags

    6HIS, MBP, TEV, and anti-Mouse Nanobody

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available Since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pELPS-eDHFR-YFP-T2A-Renilla

    Plasmid

    #193253

    Purpose

    Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase

    Depositor

    Mark Sellmyer

    Article

    Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1

    Insert

    The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    

    Use

    Lentiviral

    Tags

    Renilla luciferase (rLuc) and yellow fluorescent …

    Expression

    Mutation

    None

    Promoter

    EF1a

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZac2.1 GfaABC1D hM3D mCherry SV40

    Plasmid

    #92284

    Purpose

    AAV DREADD expression

    Depositor

    Baljit Khakh

    Article

    Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

    Insert

    hM3D (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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