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KTK_136

Plasmid

#180520

Purpose

Empty backbone for D1.1 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pME-APEX2-mCherry

Plasmid

#188944

Purpose

Middle entry vector for Gateway cloning containing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry

Depositor

Owen Tamplin

Article

Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

Insert

mito-APEX2_p2A_APEX2-H2B_p2A_mCherry

Use

Middle entry vector for gateway (l1-l2)

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_001

Plasmid

#180517

Purpose

Empty backbone for creation of entry parts for the KTK, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_247

Plasmid

#180529

Purpose

Entry vector containing pTac promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pTac promoter

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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PU.1-S148A LZRS

Plasmid

#44633

Depositor

Ellen Rothenberg

Article

Dionne et al Dev Biol. 2005 Apr 15;280(2):448-66.

Insert

PU.1 S148A (Spi1 Mouse)

Use

Retroviral

Tags

EGFP and IRES

Expression

Mammalian

Mutation

148 mutation (S to A)

Available Since

April 22, 2013

Availability

Academic Institutions and Nonprofits only
Split PS Intein

Plasmid

#242022

Purpose

Blue-light-induced reconstitution of split mCherry. A T2A peptide is inserted at the natural split site of gp41-1.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Split PS Intein

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Cp PS Intein gal4-vp16

Plasmid

#242029

Purpose

Blue-light-controlled release of gal4-vp16 transactivator from the cytoplasm to the nucleus.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Cp PS Intein gal4-vp16

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CASTOR1-HA (D304A)

Plasmid

#237419

Purpose

Transient expression of C-terminally HA-tagged human CASTOR1 (D304A mutant)

Depositor

David Sabatini

Article

Valenstein et al Nature. 2025 Aug 20. doi: 10.1038/s41586

Insert

CASTOR1 (GATSL3 Human)

Tags

HA

Expression

Mammalian

Mutation

D304A

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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KTK_346

Plasmid

#180549

Purpose

Entry vector containing gfasPurple

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

gfasPurple

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_316

Plasmid

#180547

Purpose

Entry vector containing sfGFP

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sfGFP

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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