We narrowed to 148 results for: Syp

1. natMX-ins7

   Plasmid

   #195044

   Purpose

   pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   NatR
   

   Use

   Yeast genomic targeting

   Tags

   tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIFEX-A2aGH

   Plasmid

   #160542

   Purpose

   pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEFEX1-A2aGH

   Plasmid

   #160543

   Purpose

   pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFEX1-A2aGH

   Plasmid

   #160546

   Purpose

   pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. RCAS-Neu HA SIINFEKL

   Plasmid

   #125832

   Purpose

   Retroviral expression of rat Neu cDNA

   Depositor

   Yi Li

   Article

   Du et al Proc Natl Acad Sci U S A. 2006 Nov 14;10

   Insert

   Neu (Erbb2 Rat)
   

   Use

   Retroviral

   Tags

   2x HA and SIINFEKL

   Expression

   Mammalian

   Mutation

   Val to Glu point mutation of codon 664 and trunca…

   Promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUDP002

   Plasmid

   #103872

   Purpose

   Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Inserts

   hph
   Spcas9 D147Y P411T
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Yeast

   Mutation

   D147Y P411T

   Promoter

   Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCag FlpO-2A-Cre EV

   Plasmid

   #129419

   Purpose

   episomal expression of FlpO and Cre recombinases

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/Chick β-actin (CAG)

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCag SE (Self-excising) FlpO-2A-Cre EV

   Plasmid

   #130986

   Purpose

   episomal expression of FlpO and Cre recombinases, self excising

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Tags

   Expression

   Mammalian

   Mutation

   protamine intron added to FlpO to prevent bacteri…

   Promoter

   CMV/Chick β-actin (CAG)

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

   Plasmid

   #129421

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   TagBFP2-3Flag
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   3X Flag epitope tag

   Expression

   Mutation

   Promoter

   none (3X polyA to mitigate episomal expression)

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. MADR pDonor smFP-HA (dark) WPRE

    Plasmid

    #131007

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-HA WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    HA - 10 total HA epitope tages

    Expression

    Mammalian

    Mutation

    "dark" variant with GGG fluorphore comp…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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