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pRS416-MET25p- TID-FKBP-NLS-HA3

Plasmid

#228019

Purpose

Expresses TurboID-FKBP-NLS fusion from MET25 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

MET25

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-3HA-KanMX6

Plasmid

#228024

Purpose

To integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BASU-HA3-KanMX6

Plasmid

#227978

Purpose

To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BioID2-HA3-KanMX6

Plasmid

#227976

Purpose

To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID2

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BirA-HA3-KanMX6

Plasmid

#227974

Purpose

To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pWN165

Plasmid

#215284

Purpose

Expresses sfGFP under the Anderson J23104 promoter with an RK2 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

J23104

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV052

Plasmid

#215303

Purpose

Expresses sfGFP under rhamnose induction (RhaS + RhaR + pRhaB-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

PRhaB

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWN172

Plasmid

#215291

Purpose

Expresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

J23113

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pWN212

Plasmid

#215295

Purpose

Expresses sfGFP under the Anderson J23107 promoter with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

J23107

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pWN164

Plasmid

#215283

Purpose

Expresses sfGFP under the Anderson J23101 promoter with an RK2 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

J23101

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-mad32TAG35TAG

Plasmid

#188999

Purpose

Express madanin-1 with TAG at 32 and 35 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

madanin-1 with TAG at 32 and 35 position

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-chi28TAG31TAG

Plasmid

#188996

Purpose

Express chimadanin with TAG at 28 and 31 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

Chimadanin with TAG at 28 and 31position

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-chi31TAG

Plasmid

#188985

Purpose

Express chimadanin with TAG at 31 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

Chimadanin with TAG at 31 position

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_DUX4_KBM

Plasmid

#187800

Purpose

DUX4 with KBM (p. E414-L424 deletion) with MAC-C tag

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

DUX4-KBM (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

p. E414-L424 deletion

Promoter

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_NTRK3

Plasmid

#187796

Purpose

MAC-tagged gene expression of human NTRK3

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

NTRK3 (NTRK3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_RET

Plasmid

#187775

Purpose

MAC-tagged gene expression of human RET

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

RET (RET Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPxCas12a

Plasmid

#174675

Purpose

Expresses 6xHis-MBP tagged PxCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPxCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPrCas12a

Plasmid

#174674

Purpose

Expresses 6xHis-MBP tagged PrCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPrCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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HRP-1LC

Plasmid

#139116

Purpose

express the LC of HRP-1

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

181-346

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2T Crkl SH2SH3

Plasmid

#36404

Depositor

Nora Heisterkamp

Article

de Jong et al J Biol Chem. 1995 Sep 15;270(37):21468-7

Insert

Crkl SH2 and N-terminal SH3 domains (CRKL Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
ALIX-mNeonGreen

Plasmid

#191191

Purpose

Full-length ALIX with C-terminal mNeonGreen tag.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (PDCD6IP Human, Synthetic)

Use

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 ZoAl-ENT N-1F (R1103A)

Plasmid

#216548

Purpose

For expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cells

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement protein

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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TCARZ-CMV-GFP-PAmin-GF3-R4A22

Plasmid

#203768

Purpose

For production of template RNA for use in PRINT, GFP under CMV promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TCARZ-CMV-Cherry-PAmin-GF3-R4A22

Plasmid

#203770

Purpose

For production of template RNA for use in PRINT, mCherry under CMV promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for mCherry

Use

Ivt template

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-HA_WT RAD23B

Plasmid

#201459

Purpose

Expresses human RAD23B with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FLT3-D835Y-MSCV-IRES-GFP

Plasmid

#184780

Purpose

Expresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cells

Depositor

Timothy Ley

Insert

FLT3 (FLT3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Activating tyrosine kinase domain mutation (D835Y)

Promoter

MSCV

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta K85A pcDNA3

Plasmid

#14755

Depositor

Jim Woodgett

Article

Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

Insert

GSK3 beta K85A (GSK3B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K85A. Kinase dead mutant.

Promoter

Available since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
TCARZ-CBh-GFP-SV40PA-GF3-R4A22

Plasmid

#203767

Purpose

For production of template RNA for use in PRINT, GFP under CBh promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Tags

Expression

Mutation

Promoter

CBh

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-HA_WT POLK

Plasmid

#201584

Purpose

Expresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

Polymerase kappa (POLK Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR iRFP-ZtSH2

Plasmid

#188627

Purpose

Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

ZtSH2

Use

Lentiviral

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMVV099

Plasmid

#215305

Purpose

Expresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

PCymRC

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES-Puro-TCPTP

Plasmid

#33366

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

TCPTP-45 (PTPN2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pIRES2-EGFP Mer

Plasmid

#14998

Depositor

Raymond Birge

Article

Wu et al J Cell Sci. 2005 Feb 1. 118(Pt 3):539-53

Insert

Mer (Mertk Mouse)

Use

Tags

IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
hTPST2

Plasmid

#11253

Depositor

Hyeryun Choe

Article

Choe et al Cell. 2003 Jul 25. 114(2):161-70.

Insert

TPST2 (TPST2 Human)

Use

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
hTPST1

Plasmid

#11252

Depositor

Hyeryun Choe

Article

Choe et al Cell. 2003 Jul 25. 114(2):161-70.

Insert

TPST1 (TPST1 Human)

Use

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
MAC_C_LMTK3

Plasmid

#187773

Purpose

MAC-tagged gene expression of human LMTK3

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

LMTK3 (LMTK3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TCARZ-SV40-GFP-PAmin-GF3-R4A22

Plasmid

#203769

Purpose

For production of template RNA for use in PRINT, GFP under SV40 promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Tags

Expression

Mutation

Promoter

SV40

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV SOCS-3

Plasmid

#11486

Depositor

Ronald Kahn

Article

Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

Insert

SOCS-3 (Socs3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pEFmEGFP-mDia2

Plasmid

#25407

Depositor

Arthur Alberts

Article

Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

Insert

mDia2 (Diaph3 Mouse)

Use

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

Encodes mDia2

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pMVV207

Plasmid

#215343

Purpose

gRNA expression for S. stutzeri nifL deletion on an RSF1010 backbone

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

S. stutzeri nifL gRNA

Use

CRISPR and Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

J23119

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 441 - 480 of 1600 results

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