We narrowed to 1,118 results for: gibson assembly
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Plasmid#160229Purposeplaces LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal platesDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterlacAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only
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B5-2
Plasmid#111481PurposeArg-tRNA synthetaseDepositorInsertArg-tRNA synthetase
Tags6xHisExpressionBacterialAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
B6-3
Plasmid#111487PurposeSer-tRNA synthetaseDepositorInsertSer-tRNA synthetase
Tags6xHisExpressionBacterialAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B5-4
Plasmid#111483PurposeTrp-tRNA synthetaseDepositorInsertTrp-tRNA synthetase
Tags5xHisExpressionBacterialAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B6-4
Plasmid#111488PurposePhe-tRNA synthetase ADepositorInsertPhe-tRNA synthetase A
Tags6xHisExpressionBacterialMutationA81SAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B2-3
Plasmid#111470PurposeLys-tRNA synthetaseDepositorInsertLys-tRNA synthetase
Tags6xHisExpressionBacterialAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B2-2
Plasmid#111469PurposeThr-tRNA synthetaseDepositorInsertThr-tRNA synthetase
Tags6xHisExpressionBacterialAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B4-2
Plasmid#111476PurposeAsp-tRNA synthetaseDepositorInsertAsp-tRNA synthetase
Tags6xHisExpressionBacterialAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B5-3
Plasmid#111482PurposeCys-tRNA synthetaseDepositorInsertCys-tRNA synthetase
Tags6xHisExpressionBacterialAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B8-3
Plasmid#111495PurposeHis-tRNA synthetaseDepositorInsertHis-tRNA synthetase
Tags6xHisExpressionBacterialAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B8-1
Plasmid#111494PurposeGly-tRNA synthetase BDepositorInsertGly-tRNA synthetase B
Tags6xHisExpressionBacterialAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B8-4
Plasmid#111496PurposeGly-tRNA synthetase ADepositorInsertGly-tRNA synthetase A
ExpressionBacterialAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
B4-3
Plasmid#111477PurposeAsn-tRNA synthetaseDepositorInsertAsn-tRNA synthetase
Tags6xHisExpressionBacterialAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
TOPO Venus
Plasmid#68493PurposeTOPO construct expressing Venus for generation of GMAP-compatible geneDepositorInsertsplit Venus
UseGmapExpressionBacterialPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO SV40
Plasmid#68455PurposeTOPO construct expressing SV40 for generation of GMAP-compatible promoterDepositorInsertSimian Virus Early 40 Promoter
UseGmapExpressionBacterialPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGK:HygroR-CMV:mKO2-C_UTR
Plasmid#68481PurposeLentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistanceDepositorInsertsHygromycin Resistance
mKO2-C_UTR
UseLentiviralExpressionMammalianMutationshRNA sensor cassette "B" added to 3…PromoterCMV and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE80L SpyTag-ELP-SpyTag
Plasmid#112634PurposeTwo SpyTags linked by an elastin-like polypeptide for hydrogel formationDepositorInsertSpyTag-ELP-SpyTag
TagsHis6ExpressionBacterialMutationcentral cysteinePromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-SnoopTag-AffiHER2-SpyTag
Plasmid#216280PurposeExpresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expressionDepositorInsertSnoopTag-AffiHER2-SpyTag
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, SpyTag, and Thrombin cleavage sit…ExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-Halo-Dyskerin
Plasmid#207578PurposeCRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHaloTag DKC1
TagsHaloTagExpressionMammalianPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL3-pegRNA-attL2
Plasmid#213052PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only