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Showing: 4761 - 4800 of 8321 results

  1. pIC146

    Plasmid
    #63069
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-P for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-P, IMAGE clone 5554572 (CENPP Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63069-plasmid-map-sequence-id-104384

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  2. CBR3

    Plasmid
    #38800
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CBR3 (Cbr3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 14, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. CA13

    Plasmid
    #38924
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CA13 (CA13 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. KSP_K406

    Plasmid
    #129771
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129771-plasmid-map-sequence-id-263572

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  5. hKv2.1-DN-HA

    Plasmid
    #111530
    Purpose
    Expresses a non-conducting dominant neg in mammalian cells
    Depositor
    Federico Sesti
    Insert
    human W369C-Y384T-Kv2.1 + HA (KCNB1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    W369C-Y384T
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111530-plasmid-map-sequence-id-211674

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  6. pUAST-FOXO WT

    Plasmid
    #52982
    Purpose
    insect expression of FOXO WT
    Depositor
    Marc Montminy, Biao Wang
    Insert
    FOXO (foxo Fly)
    Use
    Tags
    HA
    Expression
    Insect
    Mutation
    Promoter
    Hsp70
    Available since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pJK276

    Plasmid
    #73366
    Purpose
    Encodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)
    Depositor
    T. J. Kappock
    Insert
    plastid omega-3 fatty acid desaturase
    Use
    Tags
    Expression
    Yeast
    Mutation
    Mutation I231F. Also insert is missing residues 1…
    Promoter
    GAL1
    Available since
    May 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    73366-plasmid-map-sequence-id-135095

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  8. pZC08 (pFA6a-PSc-6xGly-PrtA-HphMX)

    Plasmid
    #44078
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44078-plasmid-map-sequence-id-61691

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  9. pTF269 (pFA6a-PSc-6xGly-12xHis-KanMX)

    Plasmid
    #44087
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44087-plasmid-map-sequence-id-61673

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  10. p-dCas9-SET7-Hygro

    Plasmid
    #104407
    Purpose
    transient expression of dCas9-SET7 fusion protein
    Depositor
    Stephan Beck
    Insert
    SET7 (SETD7 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104407-plasmid-map-sequence-id-204449

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  11. pTF278 (pFA6a-PSc-6xGly-2xStrep-KanMX)

    Plasmid
    #44088
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    2xStrep tag, 6xGly linker, ADH1 transcription ter…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44088-plasmid-map-sequence-id-61682

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  12. pTF273 (pFA6a-PSc-6xGly-12xHis-HphMX)

    Plasmid
    #44075
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44075-plasmid-map-sequence-id-61677

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  14. SOCS6

    Plasmid
    #38871
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SOCS6 (SOCS6 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    V368D, R463G
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. USP37

    Plasmid
    #36898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP37 (USP37 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 4-125 only
    Promoter
    Available since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pHH0103 APBB3 WW domain

    Plasmid
    #104262
    Purpose
    Bacterial expression of WW domain from APBB3
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    APBB3 WW domain (APBB3 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104262-plasmid-map-sequence-id-203722

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  17. pHH0103 APBB2 WW domain

    Plasmid
    #104257
    Purpose
    Bacterial expression of WW domain from APBB2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    APBB2 WW domain (APBB2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104257-plasmid-map-sequence-id-201510

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  18. pZC04 (pFA6a-PSc-6xGly-PrtA-His3MX)

    Plasmid
    #44066
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44066-plasmid-map-sequence-id-61687

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  19. pTF263 (pFA6a-TEV-6xGly-12xHis-His3MX)

    Plasmid
    #44069
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44069-plasmid-map-sequence-id-61666

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  20. pTF276 (pFA6a-PSc-6xGly-2xStrep-His3MX)

    Plasmid
    #44064
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    2xStrep tag, 6xGly linker, ADH1 transcription ter…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44064-plasmid-map-sequence-id-61680

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  21. pUASp YFP Rholike DN

    Plasmid
    #52240
    Purpose
    Drosophila expression of YFP-Rholike dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Rholike (RhoL Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T25N; DN mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  22. pAc.1B-EGFP-DmHrb98

    Plasmid
    #66595
    Purpose
    Expresses EGFP-tagged Hrb98 in Drosophila cells
    Depositor
    Elisa Izaurralde
    Insert
    Hrb98 (Hrb98DE Fly)
    Use
    Drosophila expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Actin 5C
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66595-plasmid-map-sequence-id-113871

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  23. pCRII-Topo Lhx2

    Plasmid
    #45873
    Depositor
    Jeffrey Macklis
    Insert
    Lhx2 in situ probe (Lhx2 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    contains bp# 1809-2217 of NM_010710.3
    Promoter
    Available since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  24. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

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  25. pLV-Enh eGFP Reporter-muEDC-Int3

    Plasmid
    #59288
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intronic region of the mouse Epidermal Differentiation Complex.
    Depositor
    Benjamin Yu
    Insert
    mFlg int-Intron 1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. pPyCAG-Nanog(Y136A)-IP

    Plasmid
    #62995
    Purpose
    Expresses Mouse Nanog Y136A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y136A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 136 (137 AA in Mouse Protein) to…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62995-plasmid-map-sequence-id-104597

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  27. p14-9VNA5

    Plasmid
    #50931
    Purpose
    Expresses human SRP14A5 and SRP9 proteins as a fusion protein in mammalian cells.
    Depositor
    Katharina Strub
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Use
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Mutation
    Amino acids 96-100 of SRP14 changed to alanine
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pZC05 (pFA6a-TEV-6xGly-PrtA-KanMX)

    Plasmid
    #44096
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44096-plasmid-map-sequence-id-61688

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  29. CSNK1G2

    Plasmid
    #39150
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CSNK1G2 (CSNK1G2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. pIRES-Neo-HA-cdc25C1

    Plasmid
    #40966
    Depositor
    Olivier Gavet
    Insert
    cdc25C5 (CDC25C Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. pZC06 (pFA6a-PSc-6xGly-PrtA-KanMX)

    Plasmid
    #44090
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44090-plasmid-map-sequence-id-61689

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  33. Lmx1a-in situ probe

    Plasmid
    #45071
    Depositor
    Kathleen Millen
    Insert
    Lmx1a LIM homeobox transcription factor 1 alpha (residues 94-1149) (Lmx1a Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lmx1a residues 94-1149
    Promoter
    Available since
    May 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid
    #50626
    Purpose
    pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50626-plasmid-map-sequence-id-79595

    Enlarge

  35. SLC9A3R2

    Plasmid
    #38948
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SLC9A3R2 (NHERF2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. pZC07 (pFA6a-TEV-6xGly-PrtA-HphMX)

    Plasmid
    #44084
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44084-plasmid-map-sequence-id-61690

    Enlarge

  39. pUASP Flag Rab32 WT

    Plasmid
    #44833
    Depositor
    Matthew Scott
    Insert
    Rab32 (Rab32 Fly)
    Use
    Drosophila
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. ENOSF1

    Plasmid
    #39104
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ENOSF1 (ENOSF1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 4761 - 4800 of 8321 results