We narrowed to 4,232 results for: pCMV

1. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

   Plasmid

   #140005

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

   Plasmid

   #197502

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

   Promoter

   CMV and T7

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

   Plasmid

   #197501

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

   Promoter

   CMV and T7

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

   Plasmid

   #223113

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

   Plasmid

   #79620

   Purpose

   Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Tags

   PmCDA1 and UGI

   Expression

   Mammalian

   Mutation

   D10A for nickase Cas9

   Promoter

   pCMV

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

   Plasmid

   #197507

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…

   Promoter

   CMV and T7

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

   Plasmid

   #197027

   Purpose

   Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized enRhCas12f1
   

   Expression

   Mammalian

   Mutation

   L270R

   Promoter

   CBh, CMV, hU6

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

   Plasmid

   #197500

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…

   Promoter

   CMV and T7

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

   Plasmid

   #185918

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

    Plasmid

    #197499

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

    Plasmid

    #223114

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

    Plasmid

    #197504

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-ABE8e-NRCH-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid

    #197508

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…

    Promoter

    CMV and T7

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-3XFLAG-renilla-luciferase-beta-globin-control

    Plasmid

    #184393

    Purpose

    Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR

    Plasmid

    #208379

    Purpose

    Episomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistance

    Depositor

    Daniel Hawiger

    Article

    Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    human CMV

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197026

    Purpose

    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enOsCas12f1
    

    Expression

    Mammalian

    Mutation

    D52R / T132R

    Promoter

    CBh, CMV, hU6

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-HA_N-human Prkd2 without kinase domain

    Plasmid

    #138411

    Purpose

    Express Prkd2 without kinase domain in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

    Insert

    Prkd2 without kinase domain (PRKD2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    kinase domain deleted from human Prkd2

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid

    #92390

    Purpose

    expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-TadCBEd-SpG-P2A-EGFP (BKS777/AHK571)

    Plasmid

    #223124

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpG with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpG-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpG=D10A/D1135L/S1136W/G1218…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-hAR-L26A,F27A,W433A,L436A,F437A

    Plasmid

    #89085

    Purpose

    mammalian expression of human androgen receptor with mutations of N-terminal FxxLF and WxxLF motifs

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    hAR FxxLF WxxLF mutant (AR Human)
    

    Expression

    Mammalian

    Mutation

    hAR-L26A,F27A,W433A,L436A,F437A

    Promoter

    CMV

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only