We narrowed to 26,265 results for: STI

1. pMC_mClover3_BSDminus_F1

   Plasmid

   #184166

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mClover3
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AICSDP-079:DCP1A-mEGFP

   Plasmid

   #193922

   Purpose

   Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A

   Depositor

   Allen Institute for Cell Science

   Insert

   DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pRDA_174

   Plasmid

   #136476

   Purpose

   Lentiviral expression of EnAsCas12a

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   EnAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX2T merlin 1-595

   Plasmid

   #11629

   Depositor

   Vijaya Ramesh

   Article

   Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

   Insert

   merlin (NF2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-Tol1.zf1

   Plasmid

   #61388

   Purpose

   mRNA synthesis of tol1 transposase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   tol1.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-15b-EK_C122S_His5

   Plasmid

   #49048

   Purpose

   Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

   Depositor

   Hans Brandstetter

   Article

   Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

   Insert

   Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
   

   Use

   Tags

   5xHis

   Expression

   Bacterial

   Mutation

   P92R, E185D, C122S

   Promoter

   T7

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-Cre.zf1

   Plasmid

   #61391

   Purpose

   mRNA synthesis of Cre recombinase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Cre.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pN7HELIX

   Plasmid

   #190662

   Purpose

   Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   CMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cag.Luc.puro

   Plasmid

   #74409

   Purpose

   Constitutive Luciferase expression in mammalian cells along with puromycin selection

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al EMBO Mol Med. 2017 May;9(5):672-686. doi

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAIDFA-EF1a-NmScarlet-mAID

    Plasmid

    #140615

    Purpose

    Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1 mutated F74A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-smURFP-RBS-HO-1

    Plasmid

    #80341

    Purpose

    Expresses smURFP and Heme Oxygenase-1 in bacterial cells.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    smURFP
    HO-1
    

    Use

    Tags

    6 Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-tdTomato-P2A-paCaMKII

    Plasmid

    #165431

    Purpose

    Expresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    tdTomato-P2A-paCaMKII
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-puro-SNAP-FLAG-CD59

    Plasmid

    #50374

    Purpose

    Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SR alpha

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE30-His-PheRS

    Plasmid

    #124116

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEP163

    Plasmid

    #137881

    Purpose

    PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    mRFP-T2A-BlasticidinR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a-GlyRS-His

    Plasmid

    #124110

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMDJ39 - Peft-3 Cre (PATCs)

    Plasmid

    #191381

    Purpose

    High efficiency CRE recombinase expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Peft-3 Cre tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Peft-3

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN7CAST

    Plasmid

    #190661

    Purpose

    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDonor14-AcHELIX-KanR (CJT96)

    Plasmid

    #181794

    Purpose

    pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMDJ231 - Phsp Cas9

    Plasmid

    #191382

    Purpose

    Heat shock inducible Cas9 expression in C. elegans.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Phsp-16.41 Cas9 gpd-2 tagRFP-t
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Phsp-16.41

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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