We narrowed to 34,434 results for: CaS;

1. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

   Plasmid

   #197423

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

   Plasmid

   #197424

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

   Plasmid

   #197425

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

   Plasmid

   #197426

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEN_TT 3xFLAG-BCAS2

   Plasmid

   #192294

   Purpose

   Gateway entry vector for an inducible 3xFLAG-BCAS2

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   BCAS2 (BCAS2 Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

   Plasmid

   #199253

   Purpose

   Expression construct encoding the high specificity SpCas9-KA-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity S. pyogenes Cas9-D10A-KA nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. zCREST3:Cas9green; T2_gRNA

   Plasmid

   #197647

   Purpose

   pDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   zCREST3
   Cas9
   nrg1 type II sgRNA
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. zCREST3:Cas9green; T1_gRNA

   Plasmid

   #199332

   Purpose

   pDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   zCREST3
   Cas9
   nrg1 type I sgRNA
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lenti-Cas9-sgHPRT1

   Plasmid

   #196713

   Purpose

   CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Insert

   Cas9-T2A-BSD-U6-sgHPRT1
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a/hU6

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CMV-CcuCas9

    Plasmid

    #192127

    Purpose

    Expresses CcuCas9

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    CcuCas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCas-mut4/1_2_VB

    Plasmid

    #186444

    Purpose

    Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas4 domain (K81A)

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-Hsp2Cas9

    Plasmid

    #192126

    Purpose

    Expresses Hsp2Cas9

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp2Cas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CMV-Hsp1Cas9

    Plasmid

    #192125

    Purpose

    Expresses Hsp1Cas9

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1Cas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

    Plasmid

    #195504

    Purpose

    Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG, Puro, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAS-mak10 gRNA

    Plasmid

    #160385

    Purpose

    Expresses guide RNA for targeting MAK10 in yeast

    Depositor

    John McCusker

    Insert

    mak10 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RNA pol III promoter (tRNA-Tyr)

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDuet-1_mNeongreen-Uricase

    Plasmid

    #182416

    Purpose

    Bacterial expression of mNeongreen-uricase fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    mNeongreen-uricase
    

    Use

    Tags

    mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RCAS.Sfi Akt1-K179M

    Plasmid

    #196375

    Purpose

    Akt1 kinase negative mutant K179M in avian expression vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    c-akt1 K179M
    

    Use

    Retroviral; Avian

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RCAS myr-c-P3k

    Plasmid

    #196373

    Purpose

    Oncogenic with addition of a myristylation signal and expresses in avian cells

    Depositor

    Peter Vogt

    Article

    Chang et al Science. 1997 Jun 20;276(5320):1848-50.

    Insert

    Myr-c-P3k-FLAG
    

    Use

    Retroviral; Avian

    Tags

    flag and myristoylation

    Expression

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CASK scFv [K56A/57]

    Plasmid

    #190492

    Purpose

    Mammalian Expression of CASK scFV. Derived from hybridoma K56A/57.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    CASK (Homo sapiens) recombinant scFV (CASK Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CASK scFv [K56A/50]

    Plasmid

    #190491

    Purpose

    Mammalian Expression of CASK scFV. Derived from hybridoma K56A/50.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    CASK (Homo sapiens) recombinant scFV (CASK Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge