We narrowed to 4,639 results for: crispr c plasmids

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pUASTattB-degradALFA

Plasmid

#219663

Purpose

Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

degradALFA

Expression

Insect

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-mScarlet3

Plasmid

#219640

Purpose

Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mScarlet SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-ALFA:HA

Plasmid

#219646

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

ALFA:HA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-mGreenLantern:ALFA

Plasmid

#219644

Purpose

Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mGreenLantern:ALFA SEED Cassette

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-sfGFP:ALFA

Plasmid

#219642

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP:ALFA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMC3-yeGFP

Plasmid

#176181

Purpose

yeast MoClo level-0 part plasmid type 3 containing yeast-enhanced GFP

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

yEGFP

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSEED-mGreenLantern

Plasmid

#219643

Purpose

Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mGreenLantern SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_DDX4-cterm

Plasmid

#222520

Purpose

Cas9/sgRNA plasmid for targeting DDX4

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

Cas9, DDX4 sgRNA (DDX4 Synthetic, Human)

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSEED-mScarlet3:ALFA

Plasmid

#219641

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mScarlet:ALFA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Puro-T2A-mCitrine-hSmad2-donor

Plasmid

#203663

Purpose

Donor plasmid for CRISPR knock-in

Depositor

Ali Hemmati Brivanlou

Article

Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

Insert

Smad2

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSEED-mCherry

Plasmid

#219657

Purpose

Permits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP SEED Cassette

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-N-mCherryTag

Plasmid

#218186

Purpose

To tag Hevin with mCherry at the N-terminal

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-OLLAS:HA

Plasmid

#219647

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

OLLAS:HA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Nsp2Cas9_AAV

Plasmid

#192143

Purpose

Expresses Nsp2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

Insert

Nsp2Cas9

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-GeneTRAP

Plasmid

#218184

Purpose

To KO and genetrap mouse Hevin simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-N-HATag

Plasmid

#218185

Purpose

To tag Hevin with HA at the N-terminal

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1 KO

Plasmid

#218182

Purpose

To KO Hevin in astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-Moontag:HA

Plasmid

#219648

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

MoonTag:HA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-EYFP:HA

Plasmid

#219656

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

EYFP:HA SEED Cassette

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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NarCas9_AAV

Plasmid

#192145

Purpose

Expresses NarCas9?and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

Insert

NarCas9

Use

AAV

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pYMCR2_Gb_PMLTSS

Plasmid

#140285

Purpose

CRISPR/Cas9 plasmids encoding sgRNA targeting TSS of PML for knock-in

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

Cas9

Expression

Mammalian

Promoter

CBH

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ300

Plasmid

#205414

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Ev1Cas12a without promoter

Depositor

Yiping Qi

Article

Li et al Front Genome Ed. 2023 Oct 12;5:1251903.

Insert

Ev1Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS (N terminal on insert) NLS (C terminal on ins…

Expression

Plant

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PB-5'-ENGRAM

Plasmid

#179157

Purpose

piggyBac plasmid for 5' ENGRAM recorder

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

minP

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT

Plasmid

#214041

Purpose

Bacterial expression plasmid for SAVED-CHAT from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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SlugCas9

Plasmid

#163793

Purpose

Expresses SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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SlutrCas9

Plasmid

#163795

Purpose

Expresses SlutrCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPEPX-P3-sgRNAluc

Plasmid

#85590

Purpose

Integrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template for

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

sgRNA targeting firefly luciferase encoding gene

Use

CRISPR

Expression

Bacterial

Promoter

P3

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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PEA1

Plasmid

#176527

Purpose

Delivers all prime editing nickase components in a single plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Use

CRISPR

Expression

Mammalian

Promoter

CbH for Cas9, hU6 for gRNAs

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET-21a_2xNLS_LbCpf1_protein_expression

Plasmid

#114366

Purpose

Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

Insert

2xNLS_LbCpf1

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Ago2_1

Plasmid

#73529

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only