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Plasmid#110870PurposeLentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cellsDepositorInsertxCas9(3.7)-BE3
UseLentiviralTagsExpressionMutationD10A, A262T, R324L, S409I, E480K, E543D, M694I, E…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EGFP.T2A.NCLX
Plasmid#181873PurposeExpresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoterDepositorInsertsUseAAV and AdenoviralTagsHA and mycExpressionMammalianMutationPromotersynthetic hybrid CAG promoter and synthetic hybri…Available sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-jYCaMP1
Plasmid#135422PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfectionDepositorInsertjYCaMP1
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29-hMIGA2(170-593)-6xhis
Plasmid#192834PurposeExpress codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteriaDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
UseTags6xhisExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
KSR2 gRNA (BRDN0001145237)
Plasmid#76427Purpose3rd generation lentiviral gRNA plasmid targeting human KSR2DepositorInsertKSR2 (KSR2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
KSR2 gRNA (BRDN0001147424)
Plasmid#76425Purpose3rd generation lentiviral gRNA plasmid targeting human KSR2DepositorInsertKSR2 (KSR2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
KSR2 gRNA (BRDN0001148172)
Plasmid#76426Purpose3rd generation lentiviral gRNA plasmid targeting human KSR2DepositorInsertKSR2 (KSR2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BRD3 gRNA (BRDN0001147217)
Plasmid#76094Purpose3rd generation lentiviral gRNA plasmid targeting human BRD3DepositorInsertBRD3 (BRD3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMY-CD3-P2A-CD90.2
Plasmid#163338PurposeRetroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the LTR promoterDepositorInsertCD3
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BRD3 gRNA (BRDN0001146442)
Plasmid#76092Purpose3rd generation lentiviral gRNA plasmid targeting human BRD3DepositorInsertBRD3 (BRD3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK2 gRNA (BRDN0001148866)
Plasmid#77191Purpose3rd generation lentiviral gRNA plasmid targeting human CDK2DepositorInsertCDK2 (CDK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC10A5_STOP
Plasmid#161121PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC10A5 (SLC10A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro)
Plasmid#174634PurposeOpto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)DepositorInsertKIF1A(1-365)-VVDfast-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)
UseTagsmCherry-SSPB(micro)ExpressionMammalianMutationmmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…PromoterChicken beta-actinAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL
Plasmid#119952PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
UseTagsExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…PromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SV2C_STOP
Plasmid#161463PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSV2C (SV2C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MARK4 gRNA (BRDN0001145462)
Plasmid#76727Purpose3rd generation lentiviral gRNA plasmid targeting human MARK4DepositorInsertMARK4 (MARK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-jYCaMP1s
Plasmid#135420PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variantDepositorHas ServiceAAV1InsertjYCaMP1s
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
GSK3B gRNA (BRDN0001145264)
Plasmid#77281Purpose3rd generation lentiviral gRNA plasmid targeting human GSK3BDepositorInsertGSK3B (GSK3B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMX-CAG-CD3-P2A-CD90.2
Plasmid#163336PurposeRetroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the CAG promoterDepositorInsertCD3
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
GSK3B gRNA (BRDN0001146390)
Plasmid#77282Purpose3rd generation lentiviral gRNA plasmid targeting human GSK3BDepositorInsertGSK3B (GSK3B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MARK1 gRNA (BRDN0001147433)
Plasmid#77794Purpose3rd generation lentiviral gRNA plasmid targeting human MARK1DepositorInsertMARK1 (MARK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MARK1 gRNA (BRDN0001149223)
Plasmid#77796Purpose3rd generation lentiviral gRNA plasmid targeting human MARK1DepositorInsertMARK1 (MARK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MARK4 gRNA (BRDN0001147387)
Plasmid#76728Purpose3rd generation lentiviral gRNA plasmid targeting human MARK4DepositorInsertMARK4 (MARK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD2 gRNA (BRDN0001148522)
Plasmid#75828Purpose3rd generation lentiviral gRNA plasmid targeting human CD2DepositorInsertCD2 (CD2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC25A43_STOP
Plasmid#161196PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC25A43 (SLC25A43 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC25A45_STOP
Plasmid#161220PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC25A45 (SLC25A45 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC15A5_STOP
Plasmid#161088PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC15A5 (SLC15A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-sACE2-WT(732)
Plasmid#154098PurposeMammalian expression plasmid for dimeric soluble ACE2 (untagged)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt
Plasmid#157702Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoterDepositorInsertNsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDK4 gRNA (BRDN0001145730)
Plasmid#76176Purpose3rd generation lentiviral gRNA plasmid targeting human CDK4DepositorInsertCDK4 (CDK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSK gRNA (BRDN0001149085)
Plasmid#77767Purpose3rd generation lentiviral gRNA plasmid targeting human CSKDepositorInsertCSK (CSK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAMKV gRNA (BRDN0001162514)
Plasmid#75527Purpose3rd generation lentiviral gRNA plasmid targeting human CAMKVDepositorInsertCAMKV (CAMKV Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAMKV gRNA (BRDN0001162507)
Plasmid#75528Purpose3rd generation lentiviral gRNA plasmid targeting human CAMKVDepositorInsertCAMKV (CAMKV Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K13 gRNA (BRDN0001145716)
Plasmid#76904Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K13DepositorInsertMAP3K13 (MAP3K13 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K13 gRNA (BRDN0001145899)
Plasmid#76905Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K13DepositorInsertMAP3K13 (MAP3K13 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K13 gRNA (BRDN0001148292)
Plasmid#76906Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K13DepositorInsertMAP3K13 (MAP3K13 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC38A8_STOP
Plasmid#161248PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC38A8 (SLC38A8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDK5 gRNA (BRDN0001145540)
Plasmid#77542Purpose3rd generation lentiviral gRNA plasmid targeting human CDK5DepositorInsertCDK5 (CDK5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAMKV gRNA (BRDN0001145768)
Plasmid#75526Purpose3rd generation lentiviral gRNA plasmid targeting human CAMKVDepositorInsertCAMKV (CAMKV Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC35A3_STOP
Plasmid#161301PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC35A3 (SLC35A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDK4 gRNA (BRDN0001147537)
Plasmid#76177Purpose3rd generation lentiviral gRNA plasmid targeting human CDK4DepositorInsertCDK4 (CDK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK2 gRNA (BRDN0001148950)
Plasmid#77193Purpose3rd generation lentiviral gRNA plasmid targeting human CDK2DepositorInsertCDK2 (CDK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-HsSyx3
Plasmid#179288PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + SUMO + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIG-FGFRdn
Plasmid#80431PurposeExpression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryosDepositorInsertFibroblast growth factor receptor 1 (FGFR1 Chicken)
UseTagsEGFPExpressionMammalianMutationcontains aa 1–425Promoterbeta-actin promoterAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
CD2 gRNA (BRDN0001145097)
Plasmid#75825Purpose3rd generation lentiviral gRNA plasmid targeting human CD2DepositorInsertCD2 (CD2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD2 gRNA (BRDN0001146027)
Plasmid#75826Purpose3rd generation lentiviral gRNA plasmid targeting human CD2DepositorInsertCD2 (CD2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FN3K gRNA (BRDN0001148311)
Plasmid#77432Purpose3rd generation lentiviral gRNA plasmid targeting human FN3KDepositorInsertFN3K (FN3K Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FN3K gRNA (BRDN0001148047)
Plasmid#77433Purpose3rd generation lentiviral gRNA plasmid targeting human FN3KDepositorInsertFN3K (FN3K Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FN3K gRNA (BRDN0001145506)
Plasmid#77431Purpose3rd generation lentiviral gRNA plasmid targeting human FN3KDepositorInsertFN3K (FN3K Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-FbxO11-Jeff-pcDNA3.1-
Plasmid#52503Purposeexpresses human FbxO11-Jeff mutant in mammalian cells with FLAG-HA tag at N-terminusDepositorInsertFbxO11 (FBXO11 Human)
UseTagsFLAG-HAExpressionMammalianMutationQ491L (Jeff)PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only