We narrowed to 2,641 results for: LARGE;

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pCAGEN-Dre-nls (JDW 1131)

Plasmid

#229809

Purpose

A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DRE recombinase-nls

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-I-P2A-iCre (JDW 917)

Plasmid

#224516

Purpose

A Gateway-compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet-I-P2A-iCre

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

Plasmid

#224536

Purpose

A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-FLAG- NLS-mScarlet-I3

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-mTurquoise-P2A-iCre (JDW 920)

Plasmid

#224518

Purpose

A Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mTurquoise-P2A-iCre

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-FastFUCCI (JDW 1488)

Plasmid

#242556

Purpose

Gateway middle entry clone containing FastFUCCI reporter; For visualizing cell cycle state

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

Plasmid

#229807

Purpose

A CAGGS driven tet-on transactivator with an nls-EGFP reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rtTA-IRES-nls-EGFP

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-WPRE-SV40-pA (JDW 922)

Plasmid

#224545

Purpose

A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

WPRE-SV40-pA

Use

Gateway cloning

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

Plasmid

#229828

Purpose

A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-3xNLS-mTagBFP2-Cre (JDW 918)

Plasmid

#224517

Purpose

A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xNLS-mTagBFP2-Cre

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

Plasmid

#224490

Purpose

Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Golgi-mTagBFP-P2A-H2A-iRFP

Use

Gateway cloning

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-dr-myl7-pro (JDW 1148)

Plasmid

#229836

Purpose

A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

cmlc2/myl7

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

Plasmid

#224489

Purpose

Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Sun1-2xsfGFP-6xMYC-pA

Use

Gateway cloning

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME Actin-Vhh sfGFP (JDW 1215)

Plasmid

#224495

Purpose

Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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WDV-Echistatin

Plasmid

#226985

Purpose

Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

Depositor

Wendy Gordon

Article

Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

Inserts

WDV (repA )
Echistatin

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

Plasmid

#224500

Purpose

Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mNeonGreen-HA-pA

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-lox-3xSTOP-lox (JDW 869)

Plasmid

#224521

Purpose

A Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassette

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LoxP-3xStop-LoxP

Use

Cre/Lox

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcsII-pb4-flag

Plasmid

#86768

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 4 (PSMB4 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pME-3xNLS-(G2SG2)2-Cre (JDW 1149)

Plasmid

#224514

Purpose

A Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to Cre

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xNLS-(G2SG2)2-Cre

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-TetO8x-CMVmin (JDW 1087)

Plasmid

#224475

Purpose

Gateway compatible 5' entry clone containing 8 tet operons (TREs) upstream of a minimal CMV promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TetO(8x)-CMV promoter

Use

Gateway cloning

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKTop::tse5-CT (1169-1281)

Plasmid

#192955

Purpose

For membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT (1169-1281)

Expression

Bacterial

Mutation

encodes for tse5 (1169-1281)

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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