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Plasmid#119739PurposeChemogenetic inhibitor expression plasmidDepositorInsertPSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
UseTagsExpressionMammalianMutationL131G, Q139L, Y217FPromoterCAGAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTL3
Plasmid#127966Purposeconstitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTL5
Plasmid#127967PurposeDHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4 5HT3 LC IRES GFP
Plasmid#123066PurposeChemogenetic activator expression plasmidDepositorInsertPSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
UseTagsExpressionMammalianMutationL131G Q139L Y217FPromoterCAGAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Voltron
Plasmid#119033PurposeExpress Voltron in mammalian cellsDepositorInsertVoltron
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCL.180
Plasmid#185001PurposeExpress -Eco1 AAVS1 editing ncRNA and gRNADepositorInsertEco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationAAVS1 donor, a1/a2 length extended for 27 bpPromoterH1Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
AV44_pCAG.Cas9-D10A.gRNA.S1
Plasmid#199258PurposeExpression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNADepositorInsertsSpCas9-D10A nickase
AAVS1-targeting gRNA
UseCRISPRTagsSV40 NLSExpressionMammalianMutationD10APromoterCAG promoter and RNA polymerase III promoter for …Available sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AV15_pCAG.Cas9.gRNA.S1
Plasmid#199259PurposeExpression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNADepositorInsertsSpCas9 nuclease
AAVS1-targeting gRNA
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterCAG promoter and RNA polymerase III promoter for …Available sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-Voltron-ST
Plasmid#119038PurposePan neuronal expression of soma-localized Voltron in zebrafishDepositorInsertVoltron-ST
UseZebrafish expressionTagsExpressionMutationPromoterelavl3Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Voltron-ST
Plasmid#119034PurposeExpress soma-localized Voltron in mammalian cellsDepositorInsertVoltron-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2-10xUAS-Voltron
Plasmid#119039PurposeUAS-driven expresion of Voltron in zebrafishDepositorInsertVoltron
UseZebrafish expressionTagsExpressionMutationPromoterE1bAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2-10xUAS-Voltron-ST
Plasmid#119040PurposeUAS-driven expresion of soma-localized Voltron in zebrafishDepositorInsertVoltron-ST
UseZebrafish expressionTagsExpressionMutationPromoterE1bAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-Voltron
Plasmid#119037PurposePan neuronal expression of Voltron in zebrafishDepositorInsertVoltron
UseZebrafish expressionTagsExpressionMutationPromoterelavl3Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-IVS- Syn21-Voltron-p10
Plasmid#119041PurposeUAS-driven expresion of Voltron in DrosophilaDepositorInsertVoltron
UseTagsExpressionInsectMutationPromoterhsp70Available sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDP1.2
Plasmid#171092PurposeExpresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)DepositorInsertAAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1
UseSynthetic BiologyTagsEmerald, GemininExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p13xLexAOP2-IVS-Syn21-Voltron-p10
Plasmid#119042PurposeLexAop2-driven expression of Voltron in DrosophilaDepositorInsertVoltron
UseTagsExpressionInsectMutationPromoterhsp70Available sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pInt-ERCreER
Plasmid#190040PurposeThis is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).DepositorInsertERT2-Cre-ERT2 flanked by AAVS1
UseCRISPR and TALENTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4-5HT3 HC IRES EGFP
Plasmid#119740PurposeChemogenetic activator expression plasmidDepositorUseTagsExpressionMammalianMutationL131G, Q139L, Y217F, R420Q, R424D, R428APromoterCAGAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ P (cHS4)X4 OCT4p eGFP SV40pA v2
Plasmid#115518PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.DepositorInserteGFP
UseAAV; Donor plasmid for recombinase-mediated casse…TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2
Plasmid#112668PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.DepositorInserttdT
UseAAV; Donor plasmid for recombinase-mediated casse…TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZ:F3-P CAGGS tdTPH-F
Plasmid#112667PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows constitutive expression of your protein of interest.DepositorInserttdT
UseAAV; Donor plasmid for recombinase-mediated casse…TagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2
Plasmid#112669PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.DepositorInserttdT
UseAAV; Donor plasmid for recombinase-mediated casse…TagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090
Plasmid#103869PurposedPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAVDepositorInsertsUseCRISPRTagsGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11453
Plasmid#183206PurposePB-NFKB-BFP-Triplex-crAAVS1_4-SV40pADepositorInsertCas12 Guide (crAAVS1_4)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11465
Plasmid#183623PurposePB-10X IRF-minCMV-BFP-Triplex-crAAVS1_8-SV40pADepositorInsertCas12 Guide (crAAVS1_8)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11474
Plasmid#183624PurposePB-STAT6RE-minCMV-BFP-Triplex-crAAVS1_6-SV40pADepositorInsertCas12 Guide (crAAVS1_6)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAX-SCLM
Plasmid#216757PurposeFor strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoterDepositorInsertSuperClomeleon
UseTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterCAG (cytomegalovirus-chicken beta-actin-rabbit be…Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-sgRNA
Plasmid#50662PurposeLentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)DepositorInsertsgAAVS1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK232 (CMV-OsTIR1-PURO)
Plasmid#72834PurposeExpresses OsTIR1 under the control of the CMV promoterDepositorInsertAAVS1-CMV-OsTIR1-PURO-AAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-mTurquoise2
Plasmid#191014PurposeGateway entry vector with the mTurquoise2 fluorescent proteinDepositorInsertmTurquoise2
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pLenti6.3/TO/V5-DEST-mTurquoise2
Plasmid#191010PurposeLentiviral expression vector for mTurquoise2DepositorInsertmTurquoise2
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pMK243 (Tet-OsTIR1-PURO)
Plasmid#72835PurposeExpresses OsTIR1 under the control of the Tet promoterDepositorInsertAAVS1-Tet-OsTIR1-PURO-AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGolden-Neo
Plasmid#51422PurposeContain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reactionDepositorInsertPGK1 phosphoglycerate kinase 1 (PGK1 Human)
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
PGK-EGFP-FKBPF36V
Plasmid#199765Purposeplasmid for targeting dTAG into the AAVS1 locusDepositorInsertGFP-dTAG
UseTagsGFP and dTAGExpressionMammalian and WormMutationPromoterhPGKAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMN-009
Plasmid#195413PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF1DepositorInsert4X ZF1 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRNA-U6-let7 sponge
Plasmid#35664DepositorInsertU6 let-7sponge
UseTagsExpressionMutationPromoterU6Available sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGolden-Hyg
Plasmid#51423PurposeContain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reactionDepositorInsertPGK-Hyg-pA (PGK1 Human)
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMN-018
Plasmid#195422PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF10DepositorInsert4X ZF10 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2 3xmiR-122T
Plasmid#35672DepositorInsert3 miR-122 target sites
UseLuciferaseTagsExpressionMutationPromoterHSV-TKAvailable sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
MTK234_032
Plasmid#123995PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 -hAAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_033
Plasmid#123996PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA2 -hAAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_034
Plasmid#123997PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA3 -hAAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMN-011
Plasmid#195415PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF3DepositorInsert4X ZF3 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK7_013
Plasmid#123880PurposeEncodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK systemDepositorInsertRA_hAAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMN-010
Plasmid#195414PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF2DepositorInsert4X ZF2 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMN-012
Plasmid#195416PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF4DepositorInsert4X ZF4 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMN-013
Plasmid#195417PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF5DepositorInsert4X ZF5 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMN-014
Plasmid#195418PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF6DepositorInsert4X ZF6 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only