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We narrowed to 15,859 results for: cas9

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Showing: 2461 - 2480 of 15859 results

  1. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

    Plasmid
    #84245
    Purpose
    Expresses direct fusion KRAB-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    KRAB-tagBFP-Sp dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), HA tag, KRAB, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84245-plasmid-map-sequence-id-160888

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  3. iPEmax-WT

    Plasmid
    #239385
    Purpose
    For inverse prime editor using Cas9-R221K-N394K in HEK294T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Insert
    Cas9-R221K-N393K, M-MLV-RT
    Use
    CRISPR
    Tags
    BPNLS and SV40 NLS, c-myc NLS
    Expression
    Mammalian
    Mutation
    R221K, N394K in Cas9
    Promoter
    CMV
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239385-plasmid-map-sequence-id-481781

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  5. PB-PE SpG

    Plasmid
    #179081
    Purpose
    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179081-plasmid-map-sequence-id-352652

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  7. pCY5k

    Plasmid
    #160294
    Purpose
    Yeast CRISPR plasmid targeting the kanMX cassette
    Depositor
    Amir Aharoni
    Publication
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160294-plasmid-map-sequence-id-464601

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  9. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

    Plasmid
    #84247
    Purpose
    Expresses direct fusion VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    VPR-tagBFP-Sp dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), HA tag, VPR, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84247-plasmid-map-sequence-id-160890

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  12. pUC19-E7MR72

    Plasmid
    #103105
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E7MR72(Cas9 coding gene from Solobacterium moorei F0204)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103105-plasmid-map-sequence-id-201814

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  13. pUC19-G2ZYP2

    Plasmid
    #103110
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103110-plasmid-map-sequence-id-201819

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  15. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid
    #84240
    Purpose
    Expresses GA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84240-plasmid-map-sequence-id-160812

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  19. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Publication
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    183410-plasmid-map-sequence-id-361256

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Showing: 2461 - 2480 of 15859 results