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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 2,785 results for: cdna expression plasmid

Showing: 2461 - 2480 of 2785 results

  2. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Irene Coin
    Article
    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464
    Inserts
    Humanized MbPylRS
    tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105830-plasmid-map-sequence-id-466939

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  3. cycLuc_TEVS

    Plasmid
    #119207
    Purpose
    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    cyclic luciferase with TEVs cleavage site
    Use
    Luciferase
    Tags
    6xHis and AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119207-plasmid-map-sequence-id-232048

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  8. BNLS

    Plasmid
    #50836
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP B
    Use
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50836-plasmid-map-sequence-id-76280

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  9. hACE2

    Plasmid
    #1786
    Purpose
    Mammalian expression of human ACE2
    Depositor
    Hyeryun Choe
    Article
    Li et al Nature. 2003 Nov 27. 426(6965):450-4.
    Has Service
    Cloning Grade DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    C9 (GTETSQVAPA)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 14, 2006
    Availability
    Industry, Academic Institutions, and Nonprofits
    1786-plasmid-map-sequence-id-275247

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  13. nLuc_AP10_PPVs_P9mS

    Plasmid
    #119302
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119302-plasmid-map-sequence-id-232037

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  14. nLuc_AP4_TEVs_P3mS

    Plasmid
    #119299
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
    Use
    Luciferase
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119299-plasmid-map-sequence-id-231827

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  15. AP4mS_PPVs_P3_cLuc

    Plasmid
    #119300
    Purpose
    Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119300-plasmid-map-sequence-id-232041

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  18. dCas9-NLS-gs10-cERb447

    Plasmid
    #188257
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cERb447 (ESR1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188257-plasmid-map-sequence-id-374591

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Showing: 2461 - 2480 of 2785 results