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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,632 results for: t7

Showing: 2461 - 2480 of 10632 results

  1. pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis

    Plasmid
    #246980
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged FLP recombinase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    FLP Recombinase
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    246980-plasmid-map-sequence-id-491526

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  7. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid
    #246982
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    φC31(PhiC31)
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246982-plasmid-map-sequence-id-489535

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  8. pTYB1-MBD4 pc4785

    Plasmid
    #229750
    Purpose
    Bacterial expression vector of MBD4 C-terminal tagged to the intein.
    Depositor
    Cristina Cardoso
    Insert
    MBD4 (Mbd4 Mouse)
    Tags
    Intein
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229750-plasmid-map-sequence-id-458113

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  9. pDest17_HABP4 LCD (1-413)

    Plasmid
    #235004
    Purpose
    Bacterial expression of N-terminally 6His tagged Intracellular HABP4 LCD
    Depositor
    Tanja Mittag
    Insert
    Intracellular hyaluronan-binding protein 4 (HABP4 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    235004-plasmid-map-sequence-id-468721

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  10. pDest17_AKAP8 LCD (1-367)

    Plasmid
    #235003
    Purpose
    Bacterial expression of N-terminally 6His tagged A-kinase anchor protein 8 LCD
    Depositor
    Tanja Mittag
    Insert
    A-kinase anchor protein 8 (AKAP8 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    235003-plasmid-map-sequence-id-466925

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  13. pET28-RPL3L-TwStrep

    Plasmid
    #208649
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949
    Depositor
    Quanfu Ma
    Insert
    human RPL3L (ENST00000268661) (RPL3L Human)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    contain rs753338989, rs746044959, rs752134949, pr…
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208649-plasmid-map-sequence-id-433652

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  14. pT7CFE1_polio_3ABC_C147R

    Plasmid
    #212985
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    cysteine 147 in 3C changed to arginine
    Promoter
    T7
    Available Since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212985-plasmid-map-sequence-id-422575

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  15. pT7CFE1_polio_VP12A_C109R

    Plasmid
    #212987
    Purpose
    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney VP12A C109R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    Cysteine 109 from 2A mutated to arginine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212987-plasmid-map-sequence-id-420359

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  16. pET28-RPL3-Twstrep

    Plasmid
    #208623
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) (RPL3 Human)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208623-plasmid-map-sequence-id-418316

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  17. TDRD3:JMC006-H10:C42283

    Plasmid
    #183981
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD3 in Complex with anti-TDRD3 FAB (TDRD3 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183981-plasmid-map-sequence-id-361433

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  19. pET28a-RupA_T1-NHis

    Plasmid
    #109259
    Purpose
    NRPS T domain with N-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_T1
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    contains aa 917..1025 of WP_015523502.1
    Promoter
    T7
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109259-plasmid-map-sequence-id-211504

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  20. pET28a-RupA_R-NHis

    Plasmid
    #109257
    Purpose
    NRPS R domain with N-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_R
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    contains aa 2038..2443 of WP_015523502.1
    Promoter
    T7
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109257-plasmid-map-sequence-id-211503

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Showing: 2461 - 2480 of 10632 results