We narrowed to 12,673 results for: sequence

1. pCRISPR2_wt

   Plasmid

   #149386

   Purpose

   P. aeruginosa PA14 CRISPR2 locus

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS1018-Tier1-PhCMV-p2A

   Plasmid

   #169491

   Purpose

   Tier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven 2A sequence of porcine teschovirus-1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-ccDockerin

   Plasmid

   #72200

   Purpose

   MBP-ccDockerin expression plasmid

   Depositor

   Timothy Whitehead

   Article

   Kowalsky et al Proteins. 2016 Dec;84(12):1914-1928. doi

   Insert

   Clostridium Cellulolyticum Type I Dockerin
   

   Use

   Tags

   His-Tag and Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS105

   Plasmid

   #174830

   Purpose

   Encodes a unique yeast telomere end at TEL11R by removing the subtelomere

   Depositor

   Carol Greider

   Article

   Sholes et al Genome Res. 2022 Apr;32(4):616-628. doi:

   Insert

   11R Unique
   

   Use

   Tags

   GAL4 5XUAS

   Expression

   Bacterial

   Mutation

   Changing TEL11R to a unique end

   Promoter

   lac promoter

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJJF144_SECGFP_sg2

   Plasmid

   #163865

   Purpose

   sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   sgRNA targeting GFP
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   R07E5.16 (U6)

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJJF152_Phsp_Cas9

   Plasmid

   #163862

   Purpose

   heat shock-induced expression of Cas9 in C. elegans

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   Cas9, C. elegans codon optimized with intron and SV40_NLS
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Phsp-16.48

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJJF143_SECGFP_sg1

   Plasmid

   #163864

   Purpose

   sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   sgRNA targeting GFP
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   R07E5.16 (U6)

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. plIVMD163

   Plasmid

   #160935

   Purpose

   In vivo mRNA display construct for mCherry

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

   Insert

   MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop
   

   Use

   Tags

   HIS tag, 3'UTR MS2 Stem Loop

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. EMX1*_sgRNA

   Plasmid

   #100558

   Purpose

   Expresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   EMX1 sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3_zfh1_CP-candidate_luc+

    Plasmid

    #86391

    Purpose

    Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only