Skip to main content
Addgene
Showing: 3721 - 3750 of 3980 results

  1. VinTS-tCRMod-GGSGGS5

    Plasmid
    #111763
    Purpose
    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module
    Depositor
    Brenton Hoffman
    Article
    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1
    Insert
    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Residues 1-228 of Clover, residues 1-3 deleted fr…
    Promoter
    CMV
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111763-plasmid-map-sequence-id-214717

    Enlarge

  11. pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)

    Plasmid
    #117911
    Purpose
    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM
    Depositor
    Kai Johnsson
    Article
    Yu et al Science. 2018 Sep 14;361(6407):1122-1126
    Insert
    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
    Use
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    R44A & H45Q on eDHFR
    Promoter
    T7
    Available since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    117911-plasmid-map-sequence-id-226235

    Enlarge

  18. pVITRO-Fab_Theo-LUCID

    Plasmid
    #119048
    Purpose
    Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_Theo-LUCID_part1
    Fab_Theo-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119048-plasmid-map-sequence-id-231811

    Enlarge

  19. pVITRO-Fab_Quin-LUCID

    Plasmid
    #119049
    Purpose
    Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_Quin-LUCID_part1
    Fab_Quin-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119049-plasmid-map-sequence-id-231810

    Enlarge

  20. pVITRO-Fab_MTX-LUCID

    Plasmid
    #119047
    Purpose
    Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_MTX-LUCID_part1
    Fab_MTX-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119047-plasmid-map-sequence-id-231808

    Enlarge

  25. pEND-349_Cacnes_ori

    Plasmid
    #225604
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225604-plasmid-map-sequence-id-451376

    Enlarge

  26. Actin Binding Domain Tension Sensor

    Plasmid
    #215368
    Purpose
    Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081
    Insert
    Actin Binding Domain Tension Sensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    215368-plasmid-map-sequence-id-430713

    Enlarge

Showing: 3721 - 3750 of 3980 results