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We narrowed to 11,710 results for: pcDNA

Showing: 6201 - 6250 of 11710 results

  1. pcDNA-GFP-EBA175-Flag(Y1426I)

    Plasmid
    #154976
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1426I variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    Y1426I; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154976-plasmid-map-sequence-id-309845

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  5. pcDNA5 rtTA BirA eIF4G +MIC

    Plasmid
    #158790
    Purpose
    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioID
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1 (with microexon)
    Use
    Tags
    3xFlag and BirA*
    Expression
    Mammalian
    Mutation
    Promoter
    miniCMV
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158790-plasmid-map-sequence-id-309812

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  7. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

    Plasmid
    #123340
    Purpose
    Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-EKAR-EV(T/A)
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    123340-plasmid-map-sequence-id-307135

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  10. pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)

    Plasmid
    #123344
    Purpose
    Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-CKAR(T/A)
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123344-plasmid-map-sequence-id-307096

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  11. pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

    Plasmid
    #123336
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Cerulean3-cpCerulean3-FLARE-AKAR
    Use
    Tags
    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123336-plasmid-map-sequence-id-307044

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  25. FLAG-MBP1-K295TAG-W340A-CAAX.pcDNA3-k

    Plasmid
    #127399
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Use
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: K295 to Amber codon and W34…
    Promoter
    CMV
    Available since
    July 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    127399-plasmid-map-sequence-id-248700

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  27. FLAG-MBP1-E322TAG-W340A-CAAX.pcDNA3-k

    Plasmid
    #127405
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Use
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: E322 to Amber codon and W34…
    Promoter
    CMV
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127405-plasmid-map-sequence-id-247968

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  31. pcDNA3-Flag-RNF125 Ring Mutant

    Plasmid
    #122046
    Purpose
    express RNF125 RM
    Depositor
    Ze'ev Ronai
    Insert
    RNF125 RM (RNF125 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Ring mutant C37A, C40A
    Promoter
    Available since
    June 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    122046-plasmid-map-sequence-id-242459

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  37. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

    Plasmid
    #111923
    Purpose
    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ
    Depositor
    Takeharu Nagai
    Article
    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/
    Insert
    CeNL(Ca2+)_19μ
    Use
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, F92W, E104D, D133E at CaM
    Promoter
    CMV
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111923-plasmid-map-sequence-id-222573

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  39. pcDNA5-hygro-eGFP-STOP-HEC1

    Plasmid
    #114026
    Purpose
    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.
    Depositor
    Geert Kops
    Article
    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1
    Insert
    eGFP-STOP-HEC1 (NDC80 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Start codon of Hec1 is mutated to stop codon
    Promoter
    Available since
    Sept. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    114026-plasmid-map-sequence-id-221030

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  40. CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid
    #111922
    Purpose
    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ
    Depositor
    Takeharu Nagai
    Article
    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/
    Insert
    CeNL(Ca2+)_1.2μ
    Use
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, E104D, D133E at CaM
    Promoter
    CMV
    Available since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    111922-plasmid-map-sequence-id-214013

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Showing: 6201 - 6250 of 11710 results