We narrowed to 3,069 results for: FRI

1. pCR3-FLAG-Gephyrin E

   Plasmid

   #68819

   Purpose

   Flag tagged mammalian expression of E domain

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   internal EcoRI site has silent mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pRRLsin.PPT.CMV­‐GFP­‐Eps8 WT

   Plasmid

   #74922

   Purpose

   Lentiviral plasmid expressing Eps8 fused to GFP

   Depositor

   Giorgio Scita

   Article

   Menna et al PLoS Biol. 2009 Jun 30;7(6):e1000138. do

   Insert

   Eps8 (Eps8 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

3. HNF4A_P2-135

   Plasmid

   #31067

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

   Insert

   HNF4A P2 promoter (HNF4A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   -135 to -1 of P2 HNF4A promoter

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCEE

   Plasmid

   #159746

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HNF4A_P2-226

   Plasmid

   #31064

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

   Insert

   HNF4A P2 promoter (HNF4A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   -226 to -1 of P2 HNF4A promoter

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Flag-NSF/S647A

   Plasmid

   #74938

   Purpose

   Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

   Depositor

   Elisa Greggio

   Article

   Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

   Insert

   NSF (NSF Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S647A (mutation in the D2 domain of the protein)

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

7. TH1531-DuET

   Plasmid

   #213243

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   TH1531 (FZD4 Human)
   

   Use

   Tags

   1D4 and H4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFPC2-gephyrin S268E/270E

   Plasmid

   #199609

   Purpose

   Encodes N-terminal eGFP-tagged P1-gephyrin S268/270E for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S268E/S270E

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-NDUFA10-mVenus

   Plasmid

   #185648

   Purpose

   NDUFA10 Complex I sub-unit with C-terminus mVenus tag

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   NDUFA10 (NDUFA10 Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. His-MBP-IST1 N-terminal domain

    Plasmid

    #184805

    Purpose

    Bacterial expression of IST1 N-terminal domain

    Depositor

    James Hurley

    Article

    Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    aa 1-187 only

    Promoter

    T7

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-NDUFB6-mVenus

    Plasmid

    #185652

    Purpose

    NDUFB6 Complex I sub-unit with C-terminus mVenus tag

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    NDUFB6 (NDUFB6 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-NDUFS3-mVenus

    Plasmid

    #185649

    Purpose

    NDUFS3 Complex I sub-unit with C-terminus mVenus tag

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    NDUFS3 (NDUFS3 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-NDUFA2-mVenus

    Plasmid

    #185645

    Purpose

    NDUFA2 Complex I sub-unit with C-terminus mVenus tag

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    NDUFA2 (NDUFA2 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-NDUFA10-FKBP-HA

    Plasmid

    #185810

    Purpose

    NDUFA10 subunit of Complex I fused with C-terminal FKBP and HA epitope

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    NDUFA10 (NDUFA10 Human)
    

    Use

    Lentiviral

    Tags

    FKBP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-MBP-CHMP1B, S2C

    Plasmid

    #184803

    Purpose

    Bacterial expression of CHMP1B (S2C mutation)

    Depositor

    James Hurley

    Article

    Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    S2C

    Promoter

    T7

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCUU

    Plasmid

    #159747

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1 intron 1 gRNA as2

    Plasmid

    #132394

    Purpose

    Targets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backbone

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ORAI1-YFP V107M-L273D-L276D

    Plasmid

    #114183

    Purpose

    ORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.

    Depositor

    Nicolas Demaurex

    Article

    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

    Insert

    ORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    V107M/L273D/L276D

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ORAI1-YFP T184M-L273D-L276D

    Plasmid

    #114184

    Purpose

    ORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.

    Depositor

    Nicolas Demaurex

    Article

    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

    Insert

    ORAI1-YFP T184M-L273D-L276D (ORAI1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    T184M/L273D/L276D

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 10xHis-ataxin-3 *SIM-HA

    Plasmid

    #89983

    Purpose

    Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    10xHis and HA

    Expression

    Mammalian

    Mutation

    mutated aa 162IFVV to 162AFAA

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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