We narrowed to 5,654 results for: pCas

1. BPK880-pCAG-DmrC-NLS-3xFLAG-VP64

   Plasmid

   #136912

   Purpose

   DmrC with VP64 fused to it's C-terminus; Mammalian expression vector

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

   Insert

   pCAG-DmrC-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta Actin

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

   Plasmid

   #179527

   Purpose

   For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 K233A/K234A/K253A/K263A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed lysines 233, 234, 253, and 263 to alanine

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

   Plasmid

   #166133

   Purpose

   This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

   Insert

   hU6-gRNA and hH1-gRNA targeting cldn1 gene
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-OSF-PP-squirrel-monkey-CHMP3(155)

   Plasmid

   #154183

   Purpose

   expresses squirrel monkey CHMP3(155) in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   CHMP3(155)
   

   Use

   Tags

   FLAG, Strep-Tag II, and preScission site

   Expression

   Mammalian

   Mutation

   C-terminal truncation of CHMP3 at amino acid 155

   Promoter

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

   Plasmid

   #174139

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   VRQR=D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBW2694_pCAG-PV1-pMag-L1-iCre-252C-BGHpA

   Plasmid

   #108820

   Purpose

   Expresses aa252 to C-terminus of Cre recombinase fused to pMag CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment aa252 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and pMag

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW2698_pCAG-PV1-pMag-L1-iCre-257C-BGHpA

   Plasmid

   #108822

   Purpose

   Expresses aa257 to C-terminus of Cre recombinase fused to pMag CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment aa257 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and pMag

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBW1606_pCAG-PV1-PYL-L1-PhiC31-397C-BGHpA

   Plasmid

   #108800

   Purpose

   Expresses aa397 to C-terminus of PhiC31 integrase fused to PYL CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa397 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and PYL

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBW1608_pCAG-PV1-PYL-L1-PhiC31-572C-BGHpA

   Plasmid

   #108804

   Purpose

   Expresses aa572 to C-terminus of PhiC31 integrase fused to PYL CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa572 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS and PYL

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-2/3-3'

    Plasmid

    #117325

    Purpose

    CRISPR-Cas9 for miR-124-2/3, 3'

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    gRNA miR-124-2/3-3' (MIR124-3 Synthetic)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1-3'

    Plasmid

    #117324

    Purpose

    CRISPR-Cas9 for miR-124-1, 3'

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    gRNA miR-124-1-3' (MIR124-1 Synthetic)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-3-5'

    Plasmid

    #117323

    Purpose

    CRISPR-Cas9 for miR-124-3, 5'

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    gRNA miR-124-3-5' (MIR124-3 Synthetic)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

    Plasmid

    #117322

    Purpose

    CRISPR-Cas9 for miR-124-1/2, 5'

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    gRNA miR-124-1/2-5' (MIR124-1 Synthetic)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid

    #107321

    Purpose

    Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-SaCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 (R1333S) is fused to SaCas9

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK4387 - human expression plasmid for SpCas9 Cluster 5

    Plasmid

    #101191

    Purpose

    Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster5(K918A/V922A/R925A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    K918A/V922A/R925A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MMW3645 - human expression plasmid for SpCas9 Cluster 3

    Plasmid

    #101187

    Purpose

    Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    T657A/G658A/W659A/R661A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MMW3770 - human expression plasmid for SpCas9 Cluster 4

    Plasmid

    #101189

    Purpose

    Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    F491A/M495A/T496A/N497A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. MMW2993 - human expression plasmid for SpCas9 Cluster 2

    Plasmid

    #101185

    Purpose

    Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    G582A/V583A/E584A/D585A/N588A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBW769_pCAG-PV3-FRT-loxP-iRFP720-BGHpA

    Plasmid

    #87576

    Purpose

    PV3 for 2-input, 4-output decoder circuit

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    iRFP720
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBW806_pCAG-PV3-FRT-loxP-GFP-BGHpA

    Plasmid

    #87582

    Purpose

    PV3 for 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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