Addgene

We narrowed to 3,870 results for: pes

Showing: 3751 - 3780 of 3870 results

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pCfB3048(gRNA XII-2)

Plasmid

#73290

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-2

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK2067

Plasmid

#171845

Purpose

C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)

Depositor

Judith Kimble

Article

Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:

Insert

his-58, eGFP, mCherry

Use

Tags

N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…

Expression

Worm

Mutation

boxB wild type and hairpin mutant

Promoter

mex-5 promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB3043(gRNA XI-1)

Plasmid

#73285

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3049(gRNA XII-4)

Plasmid

#73291

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgYAP-2

Plasmid

#121423

Purpose

sgYAP-2 sequence: GAGATGACTTCCTGAACAGTG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Stanley Qi, Ovijit Chaudhuri

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgYAP-2

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT (PGK) 12xHRE-NanoLuc

Plasmid

#208575

Purpose

hypoxic response element driven NanoLuc Lentiviral reporter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

NanoLuciferase
d2eGFP
12xHRE

Use

Lentiviral, Luciferase, and Synthetic Biology

Tags

3x SV40 NLS and PEST

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pOAD eIF4G1 (1-341)

Plasmid

#196833

Purpose

Expesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.

Depositor

Stan Fields

Article

Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

Insert

eIF4G1 (TIF4631 Budding Yeast)

Use

Tags

Gal4

Expression

Yeast

Mutation

amino acids 1-341

Promoter

ADH

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ

Plasmid

#192399

Purpose

Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-Rluc-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ

Plasmid

#192398

Purpose

On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-Rluc-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC

Plasmid

#192373

Purpose

To test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-TB-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

Plasmid

#192376

Purpose

To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-uORF-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

Plasmid

#192370

Purpose

To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

Plasmid

#192375

Purpose

To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-35Ster-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP729_L2_pV01_0I_FRT-OCS-rLUC_TCTP-fLUC

Plasmid

#192372

Purpose

To test for the repressive potential of the OCS with the FRT sites around it. Then used as a control to represent the off state of many cicuits.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

Plasmid

#192374

Purpose

To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCStrunc-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

Plasmid

#192385

Purpose

To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

Plasmid

#192386

Purpose

To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

Plasmid

#192404

Purpose

On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

Plasmid

#192401

Purpose

Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-Rluc-B3RT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

Plasmid

#192400

Purpose

On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-Rluc-B3RT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

Plasmid

#192402

Purpose

On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

Plasmid

#192405

Purpose

Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ

Plasmid

#192403

Purpose

Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

Plasmid

#192381

Purpose

To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

Plasmid

#192379

Purpose

To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

Plasmid

#192377

Purpose

To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

Plasmid

#192378

Purpose

To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9339 (pgRNA_VII-1_NatMX)

Plasmid

#161591

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB9340 (pgRNA_VIII-1_NatMX)

Plasmid

#161592

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB9342 (pgRNA_XIII-1_NatMX)

Plasmid

#161594

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge