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  13. peSCBE3-NG

    Plasmid
    #218163
    Purpose
    This plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218163-plasmid-map-sequence-id-433173

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  14. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Insert
    F5-flanked tetracycline resistance cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Promoter
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  15. pES134

    Plasmid
    #127551
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Article
    Wu et al Microbiology (Reading). 2024 Mar;170(3).
    Insert
    F3-flanked spectinomycin resistance cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127551-plasmid-map-sequence-id-251734

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Showing: 1 - 20 of 3901 results