We narrowed to 7,880 results for: Lif

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Unc5b.z-Fc-His

Plasmid

#72179

Purpose

Expresses the extracellular region of the Unc5B, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5b.z (Unc5b Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Plxnb1(L)-AP-His

Plasmid

#72000

Purpose

Expresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb1 (Plxnb1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3d(S)-AP-His

Plasmid

#72018

Purpose

Expresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3d (Sema3d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Neo1.f-AP-His

Plasmid

#71968

Purpose

Expresses the extracellular region of the Neogenin 1, isoform f protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.f (Neo1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Neo1.g-AP-His

Plasmid

#71969

Purpose

Expresses the extracellular region of the Neogenin 1, isoform g protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.g (Neo1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Neo1.d-AP-His

Plasmid

#71966

Purpose

Expresses the extracellular region of the Neogenin 1, isoform d protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.d (Neo1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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UAS-u(XS)Cas9

Plasmid

#127382

Purpose

Expresses Cas9 at very high level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF-Cas9

Use

CRISPR

Expression

Insect

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn NS34a-msGFP-A'Q-mito

Plasmid

#158766

Purpose

Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

NS3/4a protease and msGFP

Use

Lentiviral

Mutation

Flexible linkers between the protease and msGFP a…

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn NS34a-msGFP-'Q-mito

Plasmid

#158767

Purpose

Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

NS3/4a protease and msGFP

Use

Lentiviral

Mutation

Flexible linkers between the protease and msGFP a…

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag TMD Cys-Ala

Plasmid

#179716

Purpose

Lentiviral expression of mouse Syt7 palmitoylation site mutant with C terminal HaloTag

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pmEMBer

Plasmid

#174441

Purpose

Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

pmEMBer

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pKrox24(MapErk)DsRed

Plasmid

#200114

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

MapErk sequence in minimal promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

5 copies of designed MapErk sequence, no other pr…

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSMT3_SRSF1

Plasmid

#201056

Purpose

Express sumo-tagged human SRSF1

Depositor

Jun Zhang

Article

Fargason et al Elife. 2023 Mar 2;12:e84412. doi: 10.755

Insert

SRSF1 (SRSF1 Human)

Tags

sumo

Expression

Bacterial

Mutation

none

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

Plasmid

#100848

Purpose

AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-SRRM2-mCherry

Plasmid

#174089

Purpose

Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

Depositor

Wesley Sundquist, Katie Ullman

Article

Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

Insert

SRRM2 (SRRM2 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

TREtight

Available Since

Sept. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBbA5a-sufCDSUB

Plasmid

#195055

Purpose

Expresses SUF operon from B. subtilis from IPTG-inducible promoter

Depositor

Greg Bokinsky

Article

D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

Insert

sufCDSUB

Expression

Bacterial

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pKrox24(MapErk)dTomato

Plasmid

#200113

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

MapErk sequence in minimal promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

5 copies of designed MapErk sequence, no other pr…

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRGECO1a

Plasmid

#61563

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRGECO1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1a.WPRE.SV40

Plasmid

#100854

Purpose

AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEF5-FRT-TagRFP-T-IFT88

Plasmid

#61684

Purpose

Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

IFT88 (Ift88 Mouse)

Tags

TagRFP.T

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

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