Addgene

Showing: 2501 - 2520 of 9114 results

Results per page:

Sort results by:

pET21b-Caspase-3(D3A,V266E)

Plasmid

#90091

Purpose

Expresses caspase D3A and V266E mutant in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

Insert

Caspase 3 (CASP3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

D175A D28A D9A V266E

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3_LuxSit_i_mTagBFP2

Plasmid

#194300

Purpose

Mammalian expression of the de novo luciferase, LuxSit-i

Depositor

Andy Yeh, David Baker

Article

Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

Insert

LuxSit-i

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMuLE_ENTR_CBF-GLuc_L3-L2

Plasmid

#113868

Purpose

Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CBF (notch dependent promoter)
GLuc

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSecTag-ATX

Plasmid

#17839

Depositor

Steven Rosen

Article

Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

Insert

Autotaxin (ENPP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
ND4.2-Left TALE-G1397-N-dead-mCherry

Plasmid

#179683

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

E1347A

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-BNRF1

Plasmid

#198580

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BNRF1 (BNRF1 Epstein-Barr virus)

Use

Gateway entry vector

Tags

Expression

Mutation

None (wildtype)

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-BFRF1

Plasmid

#195801

Purpose

Gateway Entry Clone of Epstein-Barr virus BFRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BFRF1 (BFRF1 Epstein-Barr virus)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-BGLF1

Plasmid

#195818

Purpose

Gateway Entry Clone of Epstein-Barr virus BGLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BGLF1

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-BALF3

Plasmid

#195828

Purpose

Gateway Entry Clone of Epstein-Barr virus BALF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF3

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC-SARS-CoV-2 NSP3

Plasmid

#158354

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP3 (ORF1ab )

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

Plasmid

#113864

Purpose

Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

PTCH1 (hedgehog dependent promoter)
mTurquoise2

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK074 p5E exorh:EGFP

Plasmid

#195951

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:EGFP

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-BMRF1(EA-D)

Plasmid

#195807

Purpose

Gateway Entry Clone of Epstein-Barr virus BMRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BMRF1 (BMRF1 Epstein-Barr virus)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKK233-hCaM

Plasmid

#47598

Depositor

Emanuel Strehler

Article

Rhyner et al Biochemistry. 1992 Dec 29;31(51):12826-3

Insert

CaM (CALM3 Human)

Use

Tags

Expression

Bacterial

Mutation

contains 469 bp fragment (nt 92-559)

Promoter

trc

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
768 pME mApple-CAAX

Plasmid

#195961

Purpose

Gateway pME fluorophore

Depositor

Christian Mosimann, Kristen Kwan

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple-CAAX (membrane)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4.2-Left TALE-G1397-N-DddA6

Plasmid

#179680

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (Q1310R S1330I T1380I)_1xUGI_ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Q1310R, S1330I, T1380I

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-EBNALP

Plasmid

#195817

Purpose

Gateway Entry Clone of Epstein-Barr virus EBNALP

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNALP (EBNA-LP Epstein-Barr virus)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAT-WT-HA-GFP11

Plasmid

#177719

Purpose

ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112922

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
G1397 DddAtox-N–dSpCas9

Plasmid

#157835

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge