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We narrowed to 7,151 results for: CIT

Showing: 3781 - 3810 of 7151 results

  3. pαH-S-GSAS-P1-like

    Plasmid
    #173789
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein P-1-like variant (K417N)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike S-GSAS- P-1-like variant (K417N) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    173789-plasmid-map-sequence-id-340758

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  4. pDONR221-SLC25A13_STOP

    Plasmid
    #161204
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC25A13 (SLC25A13 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161204-plasmid-map-sequence-id-342379

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  5. AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid
    #65429
    Purpose
    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    ChR2(H134R)-EYFP
    Use
    AAV
    Tags
    EYFP
    Expression
    Mutation
    H134R variant
    Promoter
    hSyn1
    Available since
    July 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65429-plasmid-map-sequence-id-110864

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  8. pAAV-EF1α1.1-Chronos-tdTomato

    Plasmid
    #122099
    Purpose
    AAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    Chronos-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α (1.1 kb short version)
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122099-plasmid-map-sequence-id-242263

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  9. Pvalb-2A-Dre Flp-in replacement vector

    Plasmid
    #61571
    Purpose
    Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    Pvalb-2A-Dre (Pvalb Mouse, D6 Bacteriophage)
    Use
    Recombinase-mediated cassette exchange using flp …
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    61571-plasmid-map-sequence-id-100354

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  10. AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]

    Plasmid
    #122103
    Purpose
    AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    Chronos-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a（1.1kb short version)
    Available since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    122103-plasmid-map-sequence-id-256678

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  11. pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]

    Plasmid
    #108273
    Purpose
    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-GFP
    Use
    AAV
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1α promoter (1.1kb short version)
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    108273-plasmid-map-sequence-id-213551

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  12. pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]

    Plasmid
    #84485
    Purpose
    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    Chronos-GFP
    Use
    AAV
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α, 1.1 kb long (short version)
    Available since
    April 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    84485-plasmid-map-sequence-id-176951

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  14. pαH-S-GSAS-B.1.351.v2

    Plasmid
    #173786
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 2
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 2) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    173786-plasmid-map-sequence-id-340773

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  16. pαH-S-GSAS-B.1.351.v6

    Plasmid
    #173791
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 6
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 6) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    173791-plasmid-map-sequence-id-339984

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  17. Epac-S-H150

    Plasmid
    #170345
    Purpose
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170345-plasmid-map-sequence-id-344646

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  19. pαH-S-GSAS-B.1.351.v3

    Plasmid
    #173787
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 3
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 3) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    173787-plasmid-map-sequence-id-340760

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  20. pαH-S-GSAS-B.1.351

    Plasmid
    #171749
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-B.1.351 variant (South African)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171749-plasmid-map-sequence-id-337764

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  21. pαH-S-GSAS-D614G-K417N-E484K-N501Y

    Plasmid
    #171750
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N-E484K-N501Y
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-K417N-E484K-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171750-plasmid-map-sequence-id-337761

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  22. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165606-plasmid-map-sequence-id-330964

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  23. pαH-S-GSAS-D614G-deltaFV

    Plasmid
    #171743
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein mink variant (S-GSAS-D614G-deltaFV variant)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-deltaFV variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    171743-plasmid-map-sequence-id-337714

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  24. CROPseq-SFFV-eBFP2

    Plasmid
    #239601
    Purpose
    Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection
    Depositor
    Christian Krebs
    Article
    Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.
    Insert
    spleen focus‑forming virus - promotor
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    239601-plasmid-map-sequence-id-476817

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  25. pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122

    Plasmid
    #223184
    Purpose
    Plasmid containing the Zfr/Firefly luciferase/3xMIR122 cassette. Translation of luciferase is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.
    Depositor
    Yuxuan Guo
    Article
    Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10
    Inserts
    luciferase (LOC116160065 firefly)
    mir122 (Mir122 Mouse)
    Use
    AAV
    Tags
    HA and engineered P2A
    Expression
    Mutation
    Promoter
    TNNT2
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    223184-plasmid-map-sequence-id-443757

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Showing: 3781 - 3810 of 7151 results