We narrowed to 12,292 results for: BASE

1. G1397 DddAtox-N–SaKKH-Cas9(D10A)

   Plasmid

   #157839

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. H446 EF1a RA-ABE6.3 p2A Puro

   Plasmid

   #139104

   Purpose

   Lentiviral expression of ABE6.3.

   Depositor

   Wen Xue

   Article

   Song et al Nat Biomed Eng. 2019 Feb 25. pii: 10.103

   Insert

   ABE RA6.3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-dCpf1-BE0

   Plasmid

   #107678

   Purpose

   Expresses dLbCpf1-BE0 in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D832A/E925/D1148A

   Promoter

   CMV

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330_rActb sgRNA / hSpCas9

   Plasmid

   #172833

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330_rSyn1 sgRNA / hSpCas9

   Plasmid

   #172840

   Purpose

   Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dLbCpf1-BE0ΔiNLS

   Plasmid

   #107684

   Purpose

   Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE0ΔiNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D832A/E925A/D1148A

   Promoter

   CMV

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEN_hU6miR-G13-L

   Plasmid

   #25758

   Purpose

   Entry vector with human U6 promoter driving mouse G alpha 13 miR30-based shRNA.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   G alpha 13 miR-shRNA
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN_TmiR_G13-L_G12-E

   Plasmid

   #25763

   Purpose

   Entry vector with TRE promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   G alpha 13 and 12 miR-shRNA
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpCas9-NRCH-CGBE1

   Plasmid

   #198557

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-CGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)Gα13-125-NLuc

    Plasmid

    #207155

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNA13 (GNA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position F(125)D

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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