We narrowed to 6,905 results for: crispr cas9 plasmids

1. pSpCas9(BB)-2A-GFP-G3BP2(2)

   Plasmid

   #136061

   Purpose

   G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP2 (G3BP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS415-Cas9-VPR

   Plasmid

   #163971

   Purpose

   Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

   Depositor

   Jiazhang Lian

   Article

   Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

   Insert

   Cas9-VPR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_SpCas9_USP7 Exon 3 gRNA

   Plasmid

   #131257

   Purpose

   pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   humanised S. pyogenes Cas9 nuclease
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

   Plasmid

   #201916

   Purpose

   Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-Cas9 v2
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and Human U6

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS

   Plasmid

   #121834

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-Cas9(3.7) (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS

   Plasmid

   #121828

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7) (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. JJ802: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold

   Plasmid

   #121812

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

   Plasmid

   #121811

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. IB801: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-KRAB

   Plasmid

   #121823

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/KRAB (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

    Plasmid

    #61594

    Purpose

    A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Insert

    hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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