We narrowed to 7,366 results for: PAC

1. pAc5.1B-lambdaN-HA-DmTral-LSm-K73AD74A-V5His6_C

   Plasmid

   #146070

   Purpose

   Insect Expression of DmTral-LSm-K73AD74A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-K73AD74A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A198C compared to the sequenc…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

   Plasmid

   #146072

   Purpose

   Insect Expression of DmTral-LSm-R21EE23KS13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-R21EE23KS13AI15A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A198C compared to the sequenc…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-EGFP-DmTral-LSm-del46-60-V5His6_C

   Plasmid

   #146046

   Purpose

   Insect Expression of DmTral-LSm-del46-60

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-del46-60 (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-EGFP-DmSmaug-del584-762-V5His6_C

   Plasmid

   #146006

   Purpose

   Insect Expression of DmSmaug-del584-762

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug-del584-762 (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   7 silent mutations and a deletion of the AA 961-9…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-HA-DmSmaug-del_584-762-V5His6_C

   Plasmid

   #146015

   Purpose

   Insect Expression of DmSmaug-del584-762

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug-del584-762 (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   7 silent mutations and a deletion of the AA 961-9…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1B-lambdaN-HA-DmUPF3-K31E-siRNAres-V5His6_N

   Plasmid

   #147007

   Purpose

   Insect Expression of DmUPF3-K31E-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF3-K31E-siRNAres (Upf3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   5 silent and one non silent mutation P305S compar…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-lambdaN-HA-DmUPF1_92-1180-siRNAres-V5His6_N

   Plasmid

   #146990

   Purpose

   Insect Expression of DmUPF1_92-1180-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF1_92-1180-siRNAres (Upf1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   1 non silent mutations: G995E compared to the seq…

   Promoter

   Available since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5.1B-lambdaN-HA-DmCup_1-417-Y342AL347A-V5His6_M

   Plasmid

   #146901

   Purpose

   Insect Expression of DmCup_1-417-Y342AL347A

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

   Insert

   DmCup_1-417-Y342AL347A (cup Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACEBac1-gamma-tubulin(N229A)-TEV-His6

   Plasmid

   #178077

   Purpose

   Insect cell expression of gamma-tubulin(N229A)-TEV-His6

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

   Insert

   gamma-tubulin with an N229A point mutation (TUBG1 Human)
   

   Use

   Tags

   TEV-His6

   Expression

   Insect

   Mutation

   Changed Asparagine 229 to Alanine

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

    Plasmid

    #146071

    Purpose

    Insect Expression of DmTral-LSmR21EE23KR42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSmR21EE23KR42EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    R21E, E23K, R42E, F44A. Additionally, one silent …

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

    Plasmid

    #146049

    Purpose

    Insect Expression of DmTral-LSmR21EE23KS13AI15A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSmR21EE23KS13AI15A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S13A, I15A, R21E, E23K, one silent mutation A198C…

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1C-5Box-7-Fluc-STOP-CG10011-SV40_D

    Plasmid

    #146156

    Purpose

    Insect Expression of 5BoxB-7-Fluc-CG10011-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al RNA. 2009 Jan . 15(1):21-32.

    Insert

    5BoxB-7-Fluc-CG10011-3UTR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG

    Plasmid

    #167478

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(I539E)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG

    Plasmid

    #167479

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(C450A)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPD270 ZF43x6-Compact(overlapping ZF37)_EYFP

    Plasmid

    #138934

    Purpose

    EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Compact(overlapping ZF37)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x6-Compact(overlapping ZF37)

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

    Plasmid

    #138198

    Purpose

    FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Synthetic, Mouse)
    

    Use

    Tags

    Cerulean and cpV[E172]

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197026

    Purpose

    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enOsCas12f1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D52R / T132R

    Promoter

    CBh, CMV, hU6

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197027

    Purpose

    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enRhCas12f1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L270R

    Promoter

    CBh, CMV, hU6

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid

    #101178

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/Q695A/H698A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid

    #223065

    Purpose

    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Mutation

    Three regions of SpCas9 encode type IIS restricti…

    Promoter

    Dual T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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