Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 73,945 results for: LAS

Showing: 3811 - 3840 of 73945 results

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pTCB-trasLC

Plasmid

#237365

Purpose

pTwist CMV BetaGlobin encoding trastuzumab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

trastuzumab light chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-rituxHCknob6xHis

Plasmid

#237371

Purpose

pTwist CMV BetaGlobin encoding rituximab heavy chain with knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

rituximab heavy chain

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-cetuxHCknob6xHis

Plasmid

#237373

Purpose

pTwist CMV BetaGlobin encoding cetuximab heavy chain with knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

cetuximab heavy chain

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-magroHCLALAPGknob6xHis

Plasmid

#237375

Purpose

pTwist CMV BetaGlobin encoding magrolimab heavy chain with LALA P329G knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

magrolimab heavy chain (silent Fc)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEf1a-Dn29-dCas9-P2A-GFP

Plasmid

#247155

Purpose

Mammalian expression of a human codon optimized wildtype Dn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-dCas9-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEf1a-hifiDn29-P2A-GFP

Plasmid

#247154

Purpose

Mammalian expression of a human codon optimized engineered hifiDn29 recombinase (highest specificity variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-shControl-mCherry-CAAX

Plasmid

#227689

Purpose

Membrane tethered mCherry and scramble of shRNA targeting Atg7

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

scrambled shRNA control

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-G12-AkaLuc-P2A-Proinsulin

Plasmid

#241776

Purpose

Tested on HeLa for bioluminescence, and used for production of AAV G12-Akaluc-P2A-Proinsulin for mice.

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2025 Jul 14;16(1):6469. doi:

Insert

AkaLuc-P2A-Proinsulin

Use

AAV

Promoter

12xUAS with TATA-box minimal promoter

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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mC4-GFP

Plasmid

#221339

Purpose

Expresses murine homolog of complement 4 fused with GFP under CAG promoter

Depositor

Alberto Cruz-Martín

Article

Phadke et al Mol Psychiatry. 2025 Feb;30(2):796-809.

Insert

murine C4 fused with GFP (C4b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-nsp7

Plasmid

#168956

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp7 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_HCoV-229E-nsp1

Plasmid

#168952

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp1 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_HCoV-OC43-NS2

Plasmid

#168942

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-NS2 (ns2 )

Use

Gateway-compatible entry vector

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp15

Plasmid

#168940

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp15 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_HCoV-HKU1-Hemagglutinin-esterase

Plasmid

#168902

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-Hemagglutinin-esterase (HE )

Use

Gateway-compatible entry vector

Mutation

Codon optimized (H.sapiens)

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-nsp16

Plasmid

#168901

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp16 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_HCoV-HKU1-nsp15

Plasmid

#168900

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp15 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_SARS-CoV-2-nsp14

Plasmid

#168874

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-nsp14 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_SARS-CoV-2-nsp10

Plasmid

#168873

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-nsp10 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_SARS-CoV-1-ORF9b

Plasmid

#168860

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-ORF9b (sars9b )

Use

Gateway-compatible entry vector

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_SARS-CoV-1-nsp5

Plasmid

#168847

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-nsp5 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_MERS-CoV-Membrane

Plasmid

#168832

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-Membrane (M )

Use

Gateway-compatible entry vector

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_MERS-CoV-nsp16

Plasmid

#168826

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp16 (orf1ab )

Use

Gateway-compatible entry vector

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_MERS-CoV-nsp1

Plasmid

#168816

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp1 (orf1ab )

Use

Gateway-compatible entry vector

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanIV-Streptomyces

Plasmid

#226250

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Streptomyces)

Tags

His6

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-2C

Plasmid

#225070

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2C)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-2A

Plasmid

#225068

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2A)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-2B

Plasmid

#225069

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2B)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-57

Plasmid

#225074

Purpose

Expresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanII-57)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanIII-6

Plasmid

#225076

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanIII-6)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-23

Plasmid

#225067

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-23)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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