We narrowed to 9,586 results for: Coli

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pET21-Streptavidin-Glutamate_Tag

Plasmid

#46367

Depositor

Mark Howarth

Article

Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

Insert

Streptavidin-Glutamate_Tag

Tags

6 glutamate tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-pgaC

Plasmid

#154919

Purpose

aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting pgaC

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-periHisMBP

Plasmid

#11086

Depositor

David Waugh

Article

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Type

Empty backbone

Tags

6xHis, MBP, and MBP signal peptide

Expression

Bacterial

Promoter

Tac (lactose/IPTG inducible)

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pC28-LIC-sfGFP-His

Plasmid

#165479

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-GFP-6xHis

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pACYC-FLAG-dN6-His

Plasmid

#22143

Depositor

Robert Sauer

Article

Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

Insert

Flag-clpXdeltaNLinkedHexamer-His6

Tags

Flag, His6, and L20 Linkers

Expression

Bacterial

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pBAD-LacI

Plasmid

#46394

Purpose

Overproduction of LacI

Depositor

Florian Hollfelder

Article

Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

Insert

LacI

Tags

6xHIS tag

Expression

Bacterial

Mutation

silent T to C mutation at position 19

Promoter

arabinose promoter

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pPR220.08

Plasmid

#78553

Purpose

Plasmid constitutively expressing phycocyanobilin (PCB) biosynthesis genes and UirS sensor kinase gene

Depositor

Jeffrey Tabor

Article

Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

Insert

uirS

Use

Synthetic Biology

Promoter

J23108

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCAT112TAG-SepT

Plasmid

#34624

Depositor

Jesse Rinehart, Dieter Söll

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Insert

tRNA^Sep

Expression

Bacterial

Promoter

lpp

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pDisplay-AP-CFP-TM

Plasmid

#20861

Depositor

Alice Ting

Article

Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

AP-CFP-TM

Tags

CFP, HA, and myc

Expression

Mammalian

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

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pCas9CyMutL

Plasmid

#166981

Purpose

Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Inserts

MutL (mutL )
CymR

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Mutation

E32K

Promoter

pCymRC and pLacI

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H2_beta_CM

Plasmid

#184870

Purpose

pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

March 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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ABE-RA3.0

Plasmid

#208301

Purpose

Expresses ABE-RA3.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.0-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H1_alpha

Plasmid

#184865

Purpose

pSwap plasmid part with H1_alpha homology arms

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pETDuet-T7-Ct-YhjH

Plasmid

#79167

Purpose

Dual expression of Ct biosensor and YhjH under individual T7 promoters

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Inserts

yhjH
T7-Spinach2-Ct

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLibacceptorV2

Plasmid

#106250

Purpose

Recombination-mediated cassette exchange with target genomic loci

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP12042

Plasmid

#222354

Purpose

Tn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTAKN2_J23119-sgRNA

Plasmid

#108552

Purpose

Bacterial sgRNA expression vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

2x Streptococcus pyogenes sgRNA

Expression

Bacterial

Promoter

J23119

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pHV738

Plasmid

#117476

Purpose

Expression of NMT1

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

NMT1 (NMT1 Human)

Expression

Bacterial

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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OmpX

Plasmid

#213136

Purpose

Expresses outer membrane protein X

Depositor

Sebastian Hiller

Article

Rath et al Biochim Biophys Acta Biomembr. 2020 Jan

Insert

OmpX (24-171) (ompX E.coli (K12))

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pTKDP-neo

Plasmid

#71320

Purpose

Donor plasmid - kanamycin resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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