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Plasmid#54362DepositorTypeEmpty backboneUsePlant expressionTagsExpressionMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only
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pKmK.I4R
Plasmid#125066PurposeStorage plasmid for K.marxianus right homology armDepositorInsertRight homology arm for targeting integration to chromosome IV:240042..241741
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I4L
Plasmid#125033PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to chromosome IV:240042..241741
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
LIIIalpha fin
Plasmid#54381DepositorTypeEmpty backboneUsePlant expressionTagsExpressionMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7_circRAJ31
Plasmid#69931PurposeExpresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter.DepositorInsertpT7_circRAJ31
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
EcAvs2
Plasmid#188789PurposeAvs2 from Escherichia coli NCTC9087DepositorInsertEcAvs2
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG-B-SpyTag-C
Plasmid#87373PurposeAn ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assemblyDepositorInsertSpyTag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_19
Plasmid#64045Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPSSUB-101
Plasmid#26580DepositorInsertdestabilized GFP
UseTagsExpressionBacterialMutationS2R, C-terminal extension RPAANDENYA(LVA)PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDXNSM3
Plasmid#49028PurposeFX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), MYC, and str…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKR2.40
Plasmid#26952DepositorInsertpie-1promoter-GFP- gateway destination cassette B -pie-1 3’
UseTagsGFPExpressionWormMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT7_circRAJ31_C873U
Plasmid#69932PurposeExpresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.DepositorInsertpT7_circRAJ31_C873U
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
LIIbeta F 1-2
Plasmid#54356DepositorTypeEmpty backboneUsePlant expressionTagsExpressionMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRATIO2112
Plasmid#141296PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYCTK025
Plasmid#176729PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Lachancea mirantina (receiver part).DepositorArticleInsertste2Lm
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK028
Plasmid#176732PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Tetrapisispora phaffii (receiver part).DepositorArticleInsertste2Tp
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-134
Plasmid#185925PurposeHimar transposon delivery plasmid that delivers a orange fluorescent mOrange2 protein geneDepositorInsertmOrange2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b
Plasmid#164862PurposeInducible expression of BzCas13b nuclease in bacteria.DepositorInsertBergeyella zoohelcum Cas13b
UseTagsExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Destination Vector
Plasmid#125754PurposeDestination vector for assembling gRNA fragments in the second round of assembly.DepositorInsertpTDH3-GFPdropout-tTDH1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Guide-Generating Vector
Plasmid#125752PurposeGuide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assemblyDepositorInsertCys4-GFPdropout-SpCas9scaffold
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only