We narrowed to 9,154 results for: pho;

1. pCW-HA-hRb-delta-CDK-puro

   Plasmid

   #212673

   Purpose

   Express tagged hRb delta-CDK phosphosite mutant

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   All 15 CDK phospho-sites are mutated to alanines

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-Flag-Cdk9-IRES-eGFP

   Plasmid

   #28096

   Depositor

   Andrew Rice

   Article

   Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

   Insert

   Cdk9 (CDK9 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2011

   Availability

   Academic Institutions and Nonprofits only

3. MBP-MEK1

   Plasmid

   #68300

   Purpose

   N-terminal MBP tagged human MEK1 gene for bacterial expression

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   MBP-MEK1 (MAP2K1 Synthetic, Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSUMO1 GFP

   Plasmid

   #118360

   Purpose

   This plasmid expresses the full length human SUMO1.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hSUMO1 (SUMO1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-3myc-6His-EZH2 21D

   Plasmid

   #42664

   Depositor

   Mien-Chie Hung

   Article

   Cha et al Science. 2005 Oct 14;310(5746):306-10.

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   3Myc and 6His

   Expression

   Mammalian

   Mutation

   S21D

   Promoter

   Available since

   July 17, 2013

   Availability

   Academic Institutions and Nonprofits only

6. EGFR-ET1

   Plasmid

   #44185

   Depositor

   Timothy Springer

   Article

   Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

   Insert

   EGFR-ET1 (EGFR Human)
   

   Use

   Tags

   3C cleavge site, protein C peptide tag, his6, SBP…

   Expression

   Mammalian

   Mutation

   I104V and D254N relative to NP_005219.2

   Promoter

   CMV

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5 Flag Grb10 wt long

   Plasmid

   #37481

   Depositor

   David Sabatini

   Article

   Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

   Insert

   Grb10 (GRB10 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   isoform a, 594aa

   Promoter

   Available since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. sh-SRF

   Plasmid

   #100797

   Purpose

   expression of shRNA targeting SRF

   Depositor

   Joseph Gordon

   Article

   Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

   Insert

   rat SRF
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV HA hRB delta CDK + T373

   Plasmid

   #58912

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with T373 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pBABE-puro SHP2 E76A

    Plasmid

    #8331

    Depositor

    Ben Neel

    Article

    Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    E76A (activated mutant)

    Promoter

    Available since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-3myc-6His-EZH2 21A

    Plasmid

    #42663

    Depositor

    Mien-Chie Hung

    Article

    Cha et al Science. 2005 Oct 14;310(5746):306-10.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    3Myc and 6His

    Expression

    Mammalian

    Mutation

    S21A

    Promoter

    Available since

    July 17, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pMSCV-NLS-CBGreen-FIRE-puro

    Plasmid

    #139195

    Purpose

    Luminescent reporter of ERK activity

    Depositor

    Matthew Lazzara

    Article

    Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

    Insert

    CBG99
    

    Use

    Luciferase and Retroviral

    Tags

    Fra1 PEST domain fragment (FIRE) and nuclear loca…

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C3-PLK4 K41M-3xFLAG

    Plasmid

    #69838

    Purpose

    This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Use

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    K41M

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV HA hRB delta CDK + S612

    Plasmid

    #58914

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S612 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCMV HA hRB delta CDK + S608

    Plasmid

    #58913

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S608 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. 2XMyc-LRRK2-3XKD/Y1699C

    Plasmid

    #25367

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    "Kinase Dead" Lysine 1906 mutated to A…

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-MTS-WT-c-Src-FLAG

    Plasmid

    #44652

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Use

    Tags

    FLAG tag and mitochondria-targeting sequence (res…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKD279.8

    Plasmid

    #173171

    Purpose

    Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

    Depositor

    Jeffrey Tabor

    Article

    Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

    Inserts

    SO_4388(REC)-PsdR(DBD) 137
    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23108 and PpsdA110

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. TMEM16F-M522K-peGFP-N1

    Plasmid

    #228579

    Purpose

    Expresses mouse TMEM16F-M522K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M522K plus a 3 amino acid N-terminal truncation (…

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TMEM16F-T526K-peGFP-N1

    Plasmid

    #228580

    Purpose

    Expresses mouse TMEM16F-T526K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T526K plus a 3 amino acid N-terminal truncation (…

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge