We narrowed to 5,601 results for: Feng Zhang

1. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

   Plasmid

   #48253

   Purpose

   LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   CRY2PHR-NLS-VP64
   

   Use

   AAV; Tale

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PX398 Nitratifractor salsuginis str DSM 16511 Cas9

   Plasmid

   #68334

   Purpose

   Nitratifractor salsuginis str DSM 16511 Cas9

   Depositor

   Feng Zhang

   Insert

   NsCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pZ004 (LseC2c2 locus)

   Plasmid

   #70170

   Purpose

   Expresses LseC2c2 locus with one spacer in array

   Depositor

   Feng Zhang

   Article

   Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

   Insert

   LseC2c2 locus with one spacer in array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pU6-crRNA(SOD1)

   Plasmid

   #109321

   Purpose

   AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1

   Depositor

   Feng Zhang

   Article

   Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

   Insert

   mCherry-KASH (SOD1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn1

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pC022 - LbnCas13a from Lachnospiraceae bacterium NK4A179

   Plasmid

   #91912

   Purpose

   Expresses LbnCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LbnCas13a and guide expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115214

   Purpose

   Expresses PauCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PauCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PX382 Staphylococcus pseudintermedius Cas9

   Plasmid

   #68319

   Purpose

   Staphylococcus pseudintermedius Cas9

   Depositor

   Feng Zhang

   Insert

   SpsCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

   Plasmid

   #47451

   Purpose

   AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   TALE BB(N136_0.5NG_C63)-SID4X
   

   Use

   AAV; Tale

   Tags

   2A_phiLOV2.1

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV_hSyn_TALEBB(NI)-NLS-VP64_2A_GFP_WPRE_bGHpA

   Plasmid

   #47448

   Purpose

   AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   TALE BB(N136_0.5NI_C63)-VP64
   

   Use

   AAV; Tale

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid

    #47449

    Purpose

    AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE BB(N136_0.5NN_C63)-SID4X
    

    Use

    AAV; Tale

    Tags

    2A_phiLOV2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV_hSyn_TALEBB(NG)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid

    #47447

    Purpose

    AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE BB(N136_0.5NG_C63)-VP64
    

    Use

    AAV; Tale

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pC021 - LbmCas13a from Lachnospiraceae bacterium MA2020

    Plasmid

    #91911

    Purpose

    Expresses LbmCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LbmCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PX386 Lactobacillus farciminis Cas9

    Plasmid

    #68323

    Purpose

    Lactobacillus farciminis Cas9

    Depositor

    Feng Zhang

    Insert

    LfCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PX395 Roseburia intestinalis Cas9

    Plasmid

    #68332

    Purpose

    Roseburia intestinalis Cas9

    Depositor

    Feng Zhang

    Insert

    RiCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pC026 - PpCas13a from Paludibacter propionicigenes WB4

    Plasmid

    #91916

    Purpose

    Expresses PpCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    PpCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC024 - CgCas13a from Carnobacterium gallinarum DSM 4847

    Plasmid

    #91914

    Purpose

    Expresses CgCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    CgCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX393 Gluconacetobacter diazotrophicus Cas9

    Plasmid

    #68330

    Purpose

    Gluconacetobacter diazotrophicus Cas9

    Depositor

    Feng Zhang

    Insert

    GdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmCherry-U6-SYNPO

    Plasmid

    #140587

    Purpose

    Expresses a gRNA and mCherry in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    gRNA SYNPO
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX543 SpCas9(del175-307)

    Plasmid

    #58888

    Purpose

    Truncated form of SpCas9 without REC2 domain

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    del amino acids 175-307

    Promoter

    Available since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TALE5

    Plasmid

    #35420

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TALE5
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge