We narrowed to 30,519 results for: ica
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Plasmid#214968PurposeRed fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for better photostabilityDepositorInsertCepheid1s-ST
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightR
Plasmid#187180PurposeExpresses red norepinephrine indicator nLightR in neuronal cellsDepositorHas ServiceAAV9InsertnLightR
UseAAVTagsFlag tagExpressionMutationPromoterhuman Synapsin-1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-tadDE-PcrA M6-UGI
Plasmid#221236PurposeExpress HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.DepositorInserttadDE-PcrA M6-UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FLAG-METTL3-APPA
Plasmid#160251PurposeExpress catalytic inactive FLAG-METTL3 in human cellsDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationchanges in aa 395–398, DPPW → APPAPromoterCMVAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-pA-M.EcoGII
Plasmid#192873PurposepA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578DepositorInsertpA-M.EcoGII
UseTags3X Flag TagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6XHis-SUMO-SIRT2
Plasmid#102622PurposeExpresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purificationDepositorInsertSIRT2 (SIRT2 Human)
UseTags6XHis-SUMOExpressionBacterialMutationDeleted amino acids 1-37 and 357-389PromoterAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-dLight1.3b
Plasmid#135762PurposeFor expression of the genetically encoded dopamine sensor dLight1.3bDepositorHas ServiceAAV9InsertdLight1.3b
UseAAVTagsFlag tagExpressionMammalianMutationPromoterSynapsinAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-PcrA-UGI-T2A-mTagBFP2
Plasmid#221224PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporterDepositorInsertAID-PcrA-UGI-T2A-mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4511NES
Plasmid#138375PurposeBooster-ERK, a Red-Shifted Genetically Encoded FRET Biosensor for ERK activity.DepositorInsertBooster-ERK
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-DIO eHcKCR1 3.0-oScarlett-KV2.1
Plasmid#195198Purposeall-optical silencing methodDepositorInserteHcKCR1 3.0-oScarlet-Kv2.1
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas9::BFP-humanized
Plasmid#44247PurposeExpression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdownDepositorInsertdCas9 fused to BFP
UseCRISPR and RetroviralTags2xNLS & tagBFPExpressionMammalianMutationD10A H840A (catalytically inactive)PromoterMSCV LTR promoterAvailable sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
DRH296: FCK-Optopatch2
Plasmid#51694PurposeCoexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiologyDepositorInsertOptopatch2
UseLentiviralTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_OTmut
Plasmid#185388PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neuronsDepositorHas ServiceAAV1 and AAV9InsertGPCR activation based oxytocin control sensor GRAB_OTmut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDule2-Tet2.0
Plasmid#85497PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NEmut
Plasmid#123310PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE control sensor GRAB_NEmut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4493NES
Plasmid#138373PurposeBooster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.DepositorInsertBooster-PKA
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDule-Tet2.0
Plasmid#85496PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2-IRES-dOrai-IRES-mCherry
Plasmid#72894PurposeMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherryDepositorInsertdmBACCS2-IRES-dOrai-IRES-mCherry
UseTagsIRES-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL
Plasmid#217676PurposeAAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.DepositorInsertGtACR2-P2A-Voltron2ST
UseAAVTagsSoma targeting sequence (Kv2.1) on Voltron2 gene …ExpressionMammalianMutationPromoterEF‐1αAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUG-T2A-Pten
Plasmid#140738PurposeLentiviral vector to overexpress human PTEN and GFPDepositorInsertGFP-PTA-PTEN (PTEN Human)
UseLentiviralTagsnoneExpressionMammalianMutationPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4580NES
Plasmid#138376PurposeBooster-JNK, a Red-Shifted Genetically Encoded FRET Biosensor for JNK activity.DepositorInsertBooster-JNK
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-pSFFV-TAF15N-mCherry-sspB
Plasmid#122436PurposeExpresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.DepositorInsertTAF15 (TAF15 Human)
UseLentiviralTagsmCherry and sspBExpressionMammalianMutationContains only amino acids 1-208PromoterSFFVAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti_PGK-Cas9-P2A-HygR
Plasmid#164135PurposeLentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cellsDepositorInsertSpCas9 (cas9 Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCD4-TagRFP-T (RHPMP151)
Plasmid#119238PurposeExpresses CD4 fused to TagRFP-TDepositorInsertCD4 (CD4 Human)
UseTagstagRFP-TExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4591NES
Plasmid#138377PurposeBooster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.DepositorInsertBooster-ROCK
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_AchLightG
Plasmid#187464PurposeExpresses green acetylcholine indicator AchLightG in neuronal cellsDepositorInsertAchLightG
UseAAVTagsIgκ leaderExpressionMutationPromoterhuman Synapsin-1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_HCLS1-1/1
Plasmid#91437PurposeProtein expression and purification of human SH3 domain construct HCLS1-1/1DepositorInsertHCLS1-1/1 (HCLS1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CMV-Cas9-P2A-HygR
Plasmid#164133PurposeLentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cellsDepositorInsertSpCas9 (cas9 Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNAD1
Plasmid#154347PurposepUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistanceDepositorInsertShBle
UseMicroalgaeTagsExpressionBacterialMutationnonePromoterViolaxanthin Chlorophyll a Binding Protein Precur…Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-HypocratesCS
Plasmid#161960PurposeMammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivativesDepositorInsertHypocrates
UseTagsExpressionMammalianMutationchanged Cysteine 355 to SerinePromoterCMV, SP6Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only